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Solid State 19F NMR in ‘Model’ Membrane Systems

  • J. Post
  • Y. C. W. van der Leeuw
  • H. J. C. Berendsen
Part of the NATO Advanced Science Institutes Series book series (NSSA, volume 71)

Abstract

Solid state NMR techniques can be used to study the behaviour of molecules in membranes and their model systems1,2,3,4. We used a 4,4-difluoromyristate-D2O mixture to test the possibilities. The major interactions are the F-F dipolar coupling, the F-H dipolar coupling and the F chemical shift anisotropy. A normal 19F Free Induction Decay will be featureless and will after fourier transformation lead to a broad resonance signal (fig. 1). It can be shown, however, that a Carr-Purcell-Meiboom-Gill pulse sequence selects the F-F dipolar coupling and fourier transformation of the F.I.D. leads to a so-called Pake doublet spectrum1 (fig. 1). From the splitting the |SFF| order parameter of the fluorine pair can be directly determined, according to
$$\Delta = \left| {{S_{FF}}} \right|\Delta \left( {\rm O} \right)\Delta \left( {\rm O} \right) = 16,0 kHz$$

Keywords

Transformation Lead Bilayer Membrane Dipolar Coupling Chemical Shift Anisotropy Lyotropic Liquid Crystal 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • J. Post
    • 2
  • Y. C. W. van der Leeuw
    • 1
  • H. J. C. Berendsen
    • 1
  1. 1.Laboratory of Physical ChemistryUniversity of GroningenThe Netherlands
  2. 2.Department of Biological SciencesCarnegie Mellon Un.PittsburghUSA

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