Dynamic Interactions of Fluorescently Labeled Adenosine Deaminase in Intact Human Erythrocytes: Relation to Abnormal Activity in Malignant Cells

  • A. H. Parola
  • G. Skorka
  • P. Shuker
  • D. Gill
Part of the NATO Advanced Science Institutes Series book series (NSSA, volume 71)


Membrane modifications associated with cell transformation and malignancy are the subject of our study[1, 2]. Specifically, we explore changes due to lipid-protein and protein-protein dynamic interactions, as manifested through fluorescence depolarization of the tagged membrane component[3, 4].


Fluorescence Polarization Dansyl Chloride Photoaffinity Label Noncovalent Binding Fluorescence Depolarization 
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  1. 1.
    A. H. Parola, in: “Membranes in Tumor Growth”, T. Galeotti, ed., Elsevier, North-Holland Biomedical Press, Amsterdam, in print (1982).Google Scholar
  2. 2.
    A. H. Parola, P. W. Robbin, and E. R. Blout, Exp.Cell Res., 118: 205 (1979).PubMedCrossRefGoogle Scholar
  3. 3.
    G. Fleischer, I. Nathan, A. Livne, A. Divilansky, and A. H. Parola, in: “Platelets: Cellular Response Mechanisms and their Biological Significance”, A. Rothman, ed., John Wiley & Sons (1980).Google Scholar
  4. 4.
    I. Nathan, G. Fleischer, A. Divilansky, A. Livne, and A. H. Parola, Biochim.Biophys.Acta, 598: 417 (1980).PubMedCrossRefGoogle Scholar
  5. 5.
    R. E. Parks and R. P. Agarwal, “The Enzymes”, P. O. Boyer, ed., 3rd Edition, 7:483 (1972).Google Scholar
  6. 6.
    P. P. Trotta and M. E. Balis, Biochemistry, 17: 270 (1978).PubMedCrossRefGoogle Scholar
  7. 7.
    P. K. Chiang, G. L. Cantoni, D. A. Ray, and J. P. Bader, Biochem.Biophys.Res.Commun., 78: 336 (1977).PubMedCrossRefGoogle Scholar
  8. 8.
    R. P. Agarwal and R. E. Parks, Jr., Method Enzymol., 51: 502 (1978).CrossRefGoogle Scholar
  9. 9.
    R. P. Agarwal and R. E. Parks, Jr., Biochem.Pharmacol., 24: 547 (1975).PubMedCrossRefGoogle Scholar
  10. 10.
    C. H. M. M. de Bruyn and T. L. Oei, Adv.Exp.Med.Biol., 41A: 233 (1974).Google Scholar
  11. 11.
    M. B. Van de Weyden and W. N. Kelley, J.Biol.Chem., 251: 5418 (1976).Google Scholar
  12. 12.
    P. E. Daddona and W. N. Kelley, Biochim.Biophys.Acta, 580: 302 (1979).PubMedGoogle Scholar
  13. 13.
    M. S. Coleman, J. Donofrio, J. J. Hutton, L. Hahn, A. Daud, B. Lampkin, and J. Dyminski, J.Biol.Chem., 253: 1619 (1978).PubMedGoogle Scholar
  14. 14.
    W. Plunkett and S. S. Cohen, Cancer Res., 35: 1547 (1975).PubMedGoogle Scholar
  15. 15.
    W. P. Schroder, A. R. Stacy, and B. Pollara, J.Biol.Chem., 251: 4026 (1976).Google Scholar
  16. 16.
    J. Meier, M. S. Coleman, and J. J. Hutton, Br.J.Cancer, 33: 312 (1976).PubMedCrossRefGoogle Scholar
  17. 17.
    M. Shinitzky, Physiol.Rev., in press (1982).Google Scholar
  18. 18.
    G. Skorka, P. Shuker, D. Gill, J. Zabicky, and A. H. Parola, Biochemistry, 20: 3103 (1981).PubMedCrossRefGoogle Scholar
  19. 19.
    R. E. Holmes and R. K. Robins, J.Amer.Chem.Soc., 87: 1772 (1965).CrossRefGoogle Scholar
  20. 20.
    G. Skorka, PhD. thesis, Ben-Gurion University (1983).Google Scholar
  21. 21.
    M. Shinitzky, in this book.Google Scholar

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • A. H. Parola
    • 1
  • G. Skorka
    • 1
  • P. Shuker
    • 1
  • D. Gill
    • 1
  1. 1.Department of Chemistry and PhysicsBen-Gurion University of the NegevBeer-ShevaIsrael

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