High-Resolution Autoradiography of H3-Estradiol with Unfixed, Unembedded 1.0µ Freeze-Dried Frozen Sections

  • Walter E. Stumpf
  • Lloyd J. Roth


The autoradiographic localization of labeled compounds imposes severe limitations on the selection of procedures to which the tissue is subjected in the process. Unfortunately, this fact has not been fully recognized, and numerous studies of questionable value have been carried out with unsuitable methods. Since precise localization is the purpose of high resolution autoradiography, all steps in the procedure which are likely to cause translocation of the label must be rigidly controlled or excluded. The successful autoradiographic localization of H3-thymidine, which is incorporated into nuclear DNA, has led many people to regard a procedure which is valid in the autoradiography of incorporated thymidine as equally valid in the study of diffusible compounds.


Freeze Section Uterine Tissue Apical Pole Liquid Emulsion Autoradiographic Localization 
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Copyright information

© New England Nuclear Corporation 1968

Authors and Affiliations

  • Walter E. Stumpf
    • 1
  • Lloyd J. Roth
    • 1
  1. 1.Department of PharmacologyThe University of ChicagoChicagoUSA

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