Modification and Utilization of Isotope Fractionation Phenomena in Analytical Separations

  • Peter D. Klein
  • V. Cejka
  • Patricia A. Szczepanik


A recent review [1] of isotopic fractionation phenomena in analytical separations has shown that there has been a striking increase in the number of reports dealing with these effects in the last 4 years. Whether this increase is due to improvements in analytical techniques or to an improvement in our climate of scientific acceptability cannot be determined, but in either instance their numbers indicate that isotope fractionation is not the uncommon occurrence it was once thought to be. In particular, it has become evident that differences in the chromatographic mobilities of two isotopic species are mani­fested not only by small molecules but by substances of molecular weight 400 to 600 as well; that evidence of isotopic separations is not limited to processes of high-resolution capability; and that fractionation has been demonstrated in a wide variety of biochemical compounds.


Separation Factor Retention Volume Isotopic Substitution Chromatographic Process Silyl Ether 
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Copyright information

© New England Nuclear Corporation 1968

Authors and Affiliations

  • Peter D. Klein
    • 1
  • V. Cejka
    • 1
  • Patricia A. Szczepanik
    • 1
  1. 1.Division of Biological and Medical ResearchArgonne National LaboratoryArgonneUSA

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