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Estimation of Specific Radioactivity of Steroids Biosynthesized from Labeled Precursors

  • Kristen B. Eik-Nes

Abstract

In 1963, it was suggested that steriods containing electrophoric groups could be detected by the electron capture cell following gas-phase chromatography [1,2]. Subsequent to these publications, our laboratory observed that the chloroacetate of pure testosterone and of pure 20-reduced progesterone could be detected in submicrogram amounts by electron capture [3]. Methods were later developed for the estimation of testosterone [3], estradio[4, 5], and progesterone [6] in biological fluids utilizing electron capture of steroid chloroacetates.

Keywords

Interstitial Cell Stimulate Hormone Electron Capture Specific Radioactivity Ovarian Artery Pregnant Mare Serum Gonadotrophin 
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Copyright information

© New England Nuclear Corporation 1968

Authors and Affiliations

  • Kristen B. Eik-Nes
    • 1
  1. 1.Department of Biological ChemistryUniversity of Utah College of MedicineSalt Lake CityUSA

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