Recent Advances in the Double Isotope Derivative Analysis of Steroids

  • Bernard Kliman


The first description of the successful use of acetic anhydride-H3 for double-isotope derivative analysis of aldosterone and other steroids appeared in 1960 [1]. Since then, this methodology has been modified by many laboratories and applied to a variety of steroids in diverse biological fluids. The original methods were described in previous symposia [2, 3]. The purpose of this paper is to summarize several changes in methodology and new applications of such methods in one laboratory. Other workers have developed and applied their own modifications, which are too numerous to review here. The purpose of these changes is to provide greater reliability, speed, and sensitivity for analyses of steroids in biologic samples. The general method is applied to selective assay of specific steroids, with a range of sensitivity to 0.01 µg using 100 mc/mmole acetic anhydride-H3.


Acetic Anhydride Adrenal Vein Chromium Trioxide Cortisone Acetate Acetate Derivative 
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Copyright information

© New England Nuclear Corporation 1968

Authors and Affiliations

  • Bernard Kliman
    • 1
  1. 1.Harvard Medical SchoolBostonUSA

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