Application of Tracers to Quantitative Histochemical and Cytochemical Studies

  • Richard E. McCaman


The biochemist, concerned with metabolic characteristics of specific cell types or of specific subcellular organelles, is all too frequently limited by the sensitivity of the methods for the assay of a particular chemical or enzyme constituent. This limitation in sensitivity is a function of many variables and would include (1) the inherent sensitivity of the physical technique used for measurements (pH, absorbance, fluorescence, radioactivity, and the like), (2) the concentration or activity of the component being studied, and (3) the size of tissue sample available for study (gram vs. microgram). The latter points may apply particularly to the problems encountered in an attempt to study the enzymatic and chemical constituents of cell types present in the nervous system [1–4].


Limit Sensitivity Label Product Pyridine Nucleotide Label Substrate Cytochemical Study 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Lowry, O.H., in Waelsch, H. (editor), Biochemistry of the Developing Nervous System, Academic Press, New York, 1954.Google Scholar
  2. 2.
    Lowry, O.H., in Cohen, M., and Snider, R. (editors), Morphological and Biochemical Correlates of Neural Activity, Harper & Row, New York, 1964.Google Scholar
  3. 3.
    Robins, E., Exp. Cell Res. Suppl. 4: 241 (1957).Google Scholar
  4. 4.
    Robins, E., Smith, D.E., Eydt, K.M., and McCaman, R.E., J. Neurochem. 1: 68 (1956).CrossRefGoogle Scholar
  5. 5.
    Lowry, O.H., Roberts, N.R., Wu, M-L., Hixon, W.S., and Crawford, E.J., J. Biol. Chem. 207: 19 (1954).Google Scholar
  6. 6.
    Robins, E., Smith, D.E. and McCaman, R.E., J. Biol. Chem. 204: 927 (1953).Google Scholar
  7. 7.
    Glick, D., in Glick, D. (editor), Quantitative Chemical Techniques of Histo-and Cytochemistry, John Wiley, New York, 1961.Google Scholar
  8. 8.
    Lowry, O.H., Roberts, N.R., and Kapphahn, J.I., J.Biol. Chem. 224: 1047 (1957).Google Scholar
  9. 9.
    Lowry, O.H., Passonneau, J.V., Hasselberger, F.X., and Schulz, D.W., J. Biol. Chem. 239: 18 (1964).Google Scholar
  10. 10.
    Lowry, O.H., Passonneau, J.V., Schulz, D.W., and Rock, M.K., J. Biol. Chem. 236: 2746 (1961).Google Scholar
  11. 11.
    McCaman, R.E., McCaman, M.W., Hunt, J.M., and Smith, M.S., J. Neurochem. 12: 15 (1965).CrossRefGoogle Scholar
  12. 12.
    Axelrod, J., Albers, W., and Clemente, C.D., J. Neurochem. 5: 68 (1959).CrossRefGoogle Scholar
  13. 13.
    McCaman, R.E., Life Sci. 4: 2353 (1965).CrossRefGoogle Scholar
  14. 14.
    Axelrod, J., and Tomchick, R.J., J. Biol. Chem. 233: 702 (1958).Google Scholar
  15. 15.
    McCaman, R.E., and Cook, K., J. Biol. Chem. 241: 3390 (1966).Google Scholar
  16. 16.
    McCaman, R.E., and Cook, K., in preparation.Google Scholar
  17. 17.
    McCaman, R.E., in preparation.Google Scholar
  18. 18.
    McCaman, M.W., and McCaman, R.E., in preparation.Google Scholar
  19. 19.
    Albers, R.W., and Brady, R.O., J. Biol. Chem. 234: 926 (1959).Google Scholar
  20. 20.
    Pastan, I., Wills, V., Herring, B., and Field, J.B., J. Biol. Chem. 238: 3362 (1963).Google Scholar
  21. 21.
    Schuberth, J., Acta Chem. Scand. 17, Suppl. 1: S233 (1963).Google Scholar
  22. 22.
    McCaman, R.E., and Hunt, J.M., J. Neurochem., 12: 253 (1965).CrossRefGoogle Scholar
  23. 23.
    McCaman, R.E., McCaman, M.W., and Stafford, M.L., J. Biol. Chem. 241: 930 (1966).Google Scholar
  24. 24.
    Fuller, R.W., and Hunt, J.M., Anal. Biochem. 16: 349 (1966).CrossRefGoogle Scholar
  25. 25.
    Baldessarini, R.J., and Kopin, I.J., Anal. Biochem. 6: 289 (1963).CrossRefGoogle Scholar
  26. 26.
    Baldessarini, R.J., and Kopin, I.J., J. Neurochem. 13: 769 (1966).CrossRefGoogle Scholar
  27. 27.
    Snyder, S.H., Baldessarini, R.J., and Axelrod, J., J. Pharmacol. Exp. Therap. 153: 544 (1966).Google Scholar
  28. 28.
    Lowry, O.H., Roberts, N.R., and M-L.W. Chang, J. Biol. Chem. 222: 97 (1956).Google Scholar

Copyright information

© New England Nuclear Corporation 1968

Authors and Affiliations

  • Richard E. McCaman
    • 1
  1. 1.Institute of Psychiatric ResearchIndiana University School of MedicineIndianapolisUSA

Personalised recommendations