Methodology of Radiotracer Enzyme Assays

  • Donald J. Reed


The inherent sensitivity and specificity of radiotracer enzyme assays has resulted in a rapid increase in their development and application: Also, as additional radiochemicals become commercially available, new assay procedures are being devised. Although radiochemicals have been utilized for enzyme assays for more than a decade, no review of the methodology of these assays has been published. Therefore, an attempt has been made in this article to illustrate the general methodology, versatility, advantages, and disadvantages of radiotracer enzyme assays. Specific comparisons of these assay methods with nonisotopic assay procedures are omitted due to space limitation.


Diamine Oxidase Crude Homogenate Transferase Choline Acetyl Unreacted Substrate Cholesteryl Linoleate 
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Copyright information

© New England Nuclear Corporation 1968

Authors and Affiliations

  • Donald J. Reed
    • 1
  1. 1.Radiation Center and Department of BiochemistryBiophysics Oregon State UniversityCorvallisUSA

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