Analytical Methods: An Overview
A wide variety of analytical methods is currently being used to provide a diversity of information about proanthocyanidins. Rapid advances are being made in the ability to determine structure and conformation due to recent developments in NMR, MS, and other spectrometric techniques. In the future, these methods will play major roles in the study of conformational changes caused by associations with proteins, metals, and carbohydrates. More needs to be learned about the structure of phlobaphenes, the nature of the molecules that serve as building blocks, and the reaction mechanisms that lead to phlobaphene formation. Absence of effective chromatographic separation methods for phlobaphene components is a limiting factor. Isolations of proanthocyanidins are primarily done using low-pressure column chromatography with a variety of gel types. HPLC is presently under-utilized for such purposes, and metal-free HPLC columns need to be investigated because of the metal complexing ability of proanthocyanidins. Quantitative analysis of condensed tannins can sometimes suffer from lack of reproducibility due to details of the procedure, sample collection, and handling.
KeywordsNuclear Magnetic Resonance Condensed Tannin Nuclear Magnetic Resonance Spectroscopy Liquid Matrix Fourier Transform Mass Spectrometry
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