Pressure-Jump Studies of Ribosomal Subunit Association-Dissociation Kinetics

  • A. Wishnia
  • B. Flaig
  • F.-L. Lin


Ribosomes, the complex nucleoprotein particles on which protein synthesis occurs in all organisms, are formed from two specific subaggregates. The particles are referred to by their Standard Sedimentation coefficients: the Escherichia coli 70S ribosome, molecular weight 2.55 x 106 daltons, is composed of a small, 30S, 0.85 x 106 dalton subunit, and a large, 50S, 1.70 x 106 dalton subunit. The rates and equilibria of the 70S = 30S + 50S reaction depend markedly on the divalent metal ion concentration and less so on the Univalent metal ion concentration, as well as on temperature, etc. [1,2]. The reaction is important because a number of the reactions of protein synthesis require partial, if not total, Separation of the 30S–50S interfaces.


Protein Synthesis Pathway Adiabatic Tempera Mechanistic Impli Data Acquisition Interval Ribosome Dissociation 
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Copyright information

© Springer Science+Business Media New York 1979

Authors and Affiliations

  • A. Wishnia
    • 1
  • B. Flaig
    • 1
  • F.-L. Lin
    • 1
  1. 1.State University of New York at Stony BoookStony BrookUSA

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