Pressure-Jump Studies of Ribosomal Subunit Association-Dissociation Kinetics
Ribosomes, the complex nucleoprotein particles on which protein synthesis occurs in all organisms, are formed from two specific subaggregates. The particles are referred to by their Standard Sedimentation coefficients: the Escherichia coli 70S ribosome, molecular weight 2.55 x 106 daltons, is composed of a small, 30S, 0.85 x 106 dalton subunit, and a large, 50S, 1.70 x 106 dalton subunit. The rates and equilibria of the 70S = 30S + 50S reaction depend markedly on the divalent metal ion concentration and less so on the Univalent metal ion concentration, as well as on temperature, etc. [1,2]. The reaction is important because a number of the reactions of protein synthesis require partial, if not total, Separation of the 30S–50S interfaces.
KeywordsProtein Synthesis Pathway Adiabatic Tempera Mechanistic Impli Data Acquisition Interval Ribosome Dissociation
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