Pressure Induced Changes in the NMR Spectra of a Biopolymer
Previous studies of the effect of pressure on the helix and random coil forms of water-soluble polyamino acids by means of specific conductivity, and absorption spectroscopy in the presence of dyestuffs have been reported by K. Suzuki and Y. Taniguchi [1,2]. These authors reported the volume change for helix-to-coil transition to be negative for poly-D-glutamic acid alone and positive for this polymer in the presence of acridine orange. In order to con-firm that the helix-to-coil transition does in fact correspond to a negative volume change and that increasing pressure does favor the coil as expected by these authors, the present work has been under-taken. The polymer chosen for study, poly-N5-(3-hydroxypropyl)-L-glutamine, PHPG, has no ionizable group in its structure and therefore the helix-coil equilibrium for this polymer is wholly dependent on non-electrostatic forces.
KeywordsNuclear Magnetic Resonance Spectrum Acridine Orange Deuterium Oxide Barium Oxide Polyamino Acid
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- 6.H. L. Kliman, Ph.D. Dissertation, Princeton University, Princeton, New Jersey (1969).Google Scholar