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Method for Measuring Activation Volumes of Fast Irreversible Reactions: A High-Pressure Stopped-Flow

  • K. Heremans
  • J. Snauwaert
  • J. Rijkenberg

Abstract

Flow techniques were introduced more than fifty-years ago by Hartridge and Roughton to measure the rapid rate of oxygen binding to hemoglobin. More recently, Chance and Gibson [1] developed the stopped-flow method for the study of enzyme reactions. This method needs smaller amounts of material and therefore has become an important tool for the kinetic study of enzyme reactions and many other fast chemical reactions.

Keywords

Activation Volume Sodium Ascorbate Transient Recorder Dichlorophenol Indophenol Observation Wavelength 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    O. H. Gibson, Methods in Enzymology 16, 187 (1969).Google Scholar
  2. 2.
    M. Eigen and L. De Maeyer, Techniques of Organic Chemistry 8, 895 (1963).Google Scholar
  3. 3.
    H. Gutfreund, Ann. Rev. Biochem. 40, 315 (1971).CrossRefGoogle Scholar
  4. 4.
    K. Heremans, J. Snauwaert, H. Vandersypen, and Y. Van Nuland, in Proc. 4th Intern. Conf. High Pressure, Kyoto, Japan (1974), p. 623.Google Scholar
  5. 5.
    H. Gutfreund, Enzymes: Physical Principles, Wiley-Interscience, New York (1972), p. 179.Google Scholar
  6. 6.
    K. R. Brower, J. Am. Chem. Soc. 90, 5401 (1968).CrossRefGoogle Scholar
  7. 7.
    A. Jost, Ber. Bunsenges. physik. Chem. 80, 316 (1976).CrossRefGoogle Scholar
  8. 8.
    J. F. Below, R. E. Connick, and C. P. Coppel, J. Am. Chem. Soc. 80, 2961 (1958).CrossRefGoogle Scholar
  9. 9.
    B. B. Hasinoff, Can. J. Chem. 54, 1820 (1976).CrossRefGoogle Scholar
  10. 10.
    H. Vandersypen and K. Heremans, Arch. Int. Physiol. Biochim. 82, 792 (1974).Google Scholar
  11. 11.
    E. Stellwagen and R. D. Cass, J. Biol. Chem. 250, 2095 (1975).Google Scholar
  12. 12.
    R. C. Neuman, D. Owen and G. D. Lockyer, J. Am. Chem. Soc. 98, 2982 (1976).CrossRefGoogle Scholar
  13. 13.
    S. A. Bernhard and H. Gutfreund, Proc. Natl. Acad. Sei. (U.S.A.) 53, 1238 (1965).CrossRefGoogle Scholar
  14. 14.
    R. C. Neuman, G. D. Lockyer, and J. Marin, J. Am. Chem. Soc. 98, 6975 (1976).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1979

Authors and Affiliations

  • K. Heremans
    • 1
  • J. Snauwaert
    • 1
  • J. Rijkenberg
    • 1
  1. 1.Katholieke Universiteit te LeuvenLeuvenBelgium

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