Method for Measuring Activation Volumes of Fast Irreversible Reactions: A High-Pressure Stopped-Flow
Flow techniques were introduced more than fifty-years ago by Hartridge and Roughton to measure the rapid rate of oxygen binding to hemoglobin. More recently, Chance and Gibson  developed the stopped-flow method for the study of enzyme reactions. This method needs smaller amounts of material and therefore has become an important tool for the kinetic study of enzyme reactions and many other fast chemical reactions.
KeywordsActivation Volume Sodium Ascorbate Transient Recorder Dichlorophenol Indophenol Observation Wavelength
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