A Novel System Using the Expression of Chloramphenicol Acetyltransferase in Eukaryotic Cells Allows the Quantitative Study of Promoter Elements
As the number of isolated putative eukaryotic promoter sequences has increased, so has the need for an accurate means of measuring the function of these sequences. The in vitro transcription systems developed by Manley et al. (1980) and Weil et al. (1979) offer one approach. However, it is becoming clear that the in vitro transcription systems may respond to different regulatory signals and thus do not afford the ideal system for the study of in vivo transcriptional control (Benoist and Chambon, 1980). The study of promoters after introduction into tissue-culture cells is crucial.
KeywordsHerpes Simplex Virus Type Long Terminal Repeat Thymidine Kinase Rous Sarcoma Virus Chloramphenicol Acetyltransferase
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