The Production of Transgenic Mice
Recombinant DNA technology allows the isolation of structural genes or various portions of their associated controlling elements. These cloned sequences can then be employed in gene-transfer experiments to study the regulation of gene expression. Such gene-transfer experiments using mammalian somatic cells in culture as recipients have produced a significant body of data relating to the control of gene function (Scangos and Ruddle, 1981). This experimental approach has the limitation, however, that cultured cells are unable to undergo many of the regulatory changes required for normal embryonic development. Thus, the genetic events responsible for processes such as organ determination and tissue differentiation are difficult if not impossible to examine. Our laboratory has sought to overcome this problem by developing a gene-transfer system for the intact organism. We have chosen the mouse as a model system.
KeywordsTransgenic Mouse Germ Line Transmission Donor Material Thymidine Kinase Gene Normal Embryonic Development
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