The Production of Transgenic Mice

  • Jon W. Gordon
  • Frank H. Ruddle
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)


Recombinant DNA technology allows the isolation of structural genes or various portions of their associated controlling elements. These cloned sequences can then be employed in gene-transfer experiments to study the regulation of gene expression. Such gene-transfer experiments using mammalian somatic cells in culture as recipients have produced a significant body of data relating to the control of gene function (Scangos and Ruddle, 1981). This experimental approach has the limitation, however, that cultured cells are unable to undergo many of the regulatory changes required for normal embryonic development. Thus, the genetic events responsible for processes such as organ determination and tissue differentiation are difficult if not impossible to examine. Our laboratory has sought to overcome this problem by developing a gene-transfer system for the intact organism. We have chosen the mouse as a model system.


Transgenic Mouse Germ Line Transmission Donor Material Thymidine Kinase Gene Normal Embryonic Development 
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  1. Blin, N., and Stafford, D. W., 1976, A general method for isolation of high molecular weight DNA from eukaryotes, Nucleic Acids Res. 3: 2303–2308.PubMedCrossRefGoogle Scholar
  2. Brinster, R. L., Chen, H. Y., Trumbauer, M., Senear, A. W., Warren, R., and Palmiter, R. D., 1981, Somatic expression of herpes thymidine kinase in mice following injection of a fusion gene into eggs, Cell 27: 223–231.PubMedCrossRefGoogle Scholar
  3. Costantini, F., and Lacy, E., 1981, Introduction of a rabbit beta-globin gene into the mouse germ line, Nature (London) 294: 92–94.CrossRefGoogle Scholar
  4. Gordon, J. W., and Ruddle, F. H., 1981, Integration and stable germ line transmission of genes injected into mouse pronuclei, Science 214: 1244–1246.PubMedCrossRefGoogle Scholar
  5. Gordon, J. W., and Ruddle, F. H., 1982, Germ line transmission in transgenic mice, in: Embryonic Development, Part B, Cellular Aspects ( M. M. Burger and R. Weber, eds.), Alan R. Liss, New York, pp. 111–124.Google Scholar
  6. Gordon, J. W., Scangos, G. A., Plotkin, D. J., Barbosa, J. A., and Ruddle, F. H., 1980, Genetic transformation of mouse embryos by microinjection of purified DNA, Proc. Natl. Acad. Sci. U.S.A. 77: 7380–7384.PubMedCrossRefGoogle Scholar
  7. Harbers, K., Jahner, D., and Jaenisch, R., 1981, Microinjection of cloned retroviral genomes into mouse zygotes: Integration and expression in the animal, Nature (London) 293: 540–542.CrossRefGoogle Scholar
  8. Mantei, N., and Weissman, C., 1982, Controlled transcription of a human a-interferon gene introduced into mouse L cells, Nature (London) 297: 128–132.CrossRefGoogle Scholar
  9. McClelland, A., Gordon, J. W., and Ruddle, F. H., 1984, Molecular cloning of integrated DNA from a transgenic mouse (in preparation).Google Scholar
  10. Scangos, G. A., and Ruddle, F. H., 1981, Mechanisms and applications of DNA-mediated gene transfer in mammalian cells—a review, Gene 14: 1–10.PubMedCrossRefGoogle Scholar
  11. Southern, E. M., 1975, Detection of specific sequences among DNA fragments separated by gel electrophoresis, J. Mol. Biol. 98: 503–517.PubMedCrossRefGoogle Scholar
  12. Wagner, T. E., Hoppe, P. C., Jollick, J. D., Scholl, D. R., Hodinka, R. L., and Gault, J. B., 1981 a, Microinjection of a rabbit beta-globin gene into zygotes and its subsequent expression in adult mice and their offspring, Proc. Natl. Acad. Sci. U.S.A. 78: 6376–6380.CrossRefGoogle Scholar
  13. Wagner, E. F., Stewart, T. A., and Mintz, B., 1981b, The human beta-globin gene and a functional viral thymidine kinase gene in developing mice, Proc. Natl. Acad. Sci. U.S.A. 78: 5016–5020.PubMedCrossRefGoogle Scholar
  14. Wahl, G. M., Stern, M., and Stark, G. R., 1979, Efficient transfer of large DNA fragments from agarose gels to dioxybenzoyloxymethyl-paper and rapid hybridization using dextran sulfate, Proc. Natl. Acad. Sci. U.S.A. 76: 3683–3687.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Jon W. Gordon
    • 1
  • Frank H. Ruddle
    • 1
  1. 1.Department of BiologyYale UniversityNew HavenUSA

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