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Expression of Transferred Thymidine Kinase Genes in Mouse L Cells: Evidence for Control by DNA Methylation

  • Barbara Christy
  • George Scangos
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)

Abstract

The presence of methylated bases in the DNA of both prokaryotes and eukaryotes has been known for some time. In prokaryotic DNA, both 6-methyladenosine and 5-methylcytosine (m5C) are found, while in mammalian DNA, the only detectable methylated base is m5C (Wyatt, 1951). From 2 to 7% of cytosine residues are found as m5C, and more than 90% of the methylated C residues are found in the dinucleotide sequence CpG (Grippo et al., 1970). The distribution of m5C in the DNA is nonrandom, and both the pattern and level of methylation appear to be species-specific and tissue-specific (Razin and Riggs, 1980; Ehrlich and Wang, 1981). The pattern of methylation is probably established during development and is passed on from a differentiated cell to its daughters following the action of one or more “maintenance methylase” enzymes, which act on hemimethylated DNA shortly after replication (Gruenbaum et al., 1982). The symmetry of the CpG sequence allows one parental strand, which is methylated, to act as a template for the methylation of the new strand (Holliday and Pugh, 1975; Razin and Riggs, 1980; Ehrlich and Wang, 1981).

Keywords

Herpes Simplex Virus Thymidine Kinase Herpes Simplex Virus Thymidine Kinase Thymidine Kinase Gene Thymidine Kinase Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Behe, M., and Felsenfeld, G., 1981, Proc. Natl. Acad. Sci. U.S.A. 78: 1619–1623.PubMedCrossRefGoogle Scholar
  2. Bird, A., Taggert, M., and Macleod, D., 1981, Cell 26: 381–390.PubMedCrossRefGoogle Scholar
  3. Breznik, T., and Cohen, J. C., 1982, Nature (London) 295: 255–257.CrossRefGoogle Scholar
  4. Christy, B., and Scangos, G., 1982, Proc. Natl. Acad. Sci. U.S.A. 79: 6299–6303PubMedCrossRefGoogle Scholar
  5. Clough, D. W., Kunkel, L. M., and Davidson, R. L., 1982, Science 206: 70–73CrossRefGoogle Scholar
  6. Compere, S. J., and Palmiter, R. D., 1981, Cell 25: 233–240.PubMedCrossRefGoogle Scholar
  7. Davies, R. L., Fuhrer-Krusi, S., and Kucherlapati, R. S., 1982, Cell 31: 521–529PubMedCrossRefGoogle Scholar
  8. Ehrlich, M., and Wang, R. Y.-H., 1981, Science 212: 1350–1357.PubMedCrossRefGoogle Scholar
  9. Enquist, L. W., VandeWoude, G. F., Wagner, M., Smiley, J. R., and Summers, W. C., 1979, Gene 7: 335–342.PubMedCrossRefGoogle Scholar
  10. Grippo, P., Iacarino, M., Parisi, E., and Scarano, E., 1970, J. Mol. Biol. 35: 195–208Google Scholar
  11. Groudine, M., Eisenman, R., and Weintraub, H., 1981, Nature (London) 292: 311–317CrossRefGoogle Scholar
  12. Gruenbaum, Y., Cedar, H., and Razin, A., 1982, Nature (London) 295: 620–622.CrossRefGoogle Scholar
  13. Harbers, K., Schnieke, A., Stuhlmann, H., Jahner, D., and Jaenisch, R., 1981, Proc. Natl. Acad. Sci. U.S.A. 78: 7609–7613.PubMedCrossRefGoogle Scholar
  14. Holliday, R., and Pugh, J. E., 1975, Science 187: 226–232.PubMedCrossRefGoogle Scholar
  15. Huttner, K. M., Barbosa, J. A., Scangos, G. A., and Ruddle, F. H., 1981, J. Cell Biol. 91: 153–156.PubMedCrossRefGoogle Scholar
  16. Jones, P. A., and Taylor, S. M., 1980, Cell 20: 85–93.PubMedCrossRefGoogle Scholar
  17. Kuo, M. T., Mandel, J. L., and Chambon, P., 1979, Nucleic Acids Res. 7: 2105–2113PubMedCrossRefGoogle Scholar
  18. Landolph, J. R., and Jones, P. A., 1982, Cancer Res. 42: 817–823.PubMedGoogle Scholar
  19. Littlefield, J. W., 1964, Science 145: 709–710.PubMedCrossRefGoogle Scholar
  20. Mandel, J. L., and Chambon, P., 1979, Nucleic Acids Res. 7: 2081–2103.PubMedCrossRefGoogle Scholar
  21. McKnight, S. L., 1981, Nucleic Acids Res. 8: 5949–5964.CrossRefGoogle Scholar
  22. Ostrander, M., Vogel, S., and Silverstein, S., 1982, Mol. Cell. Biol. 2: 708–714.PubMedGoogle Scholar
  23. Pollack, Y., Stein, R., Razin, A., and Cedar, H., 1980, Proc. Natl. Acad. Sci. U.S.A. 77: 6463–6467PubMedCrossRefGoogle Scholar
  24. Razin, A., and Riggs, A. D., 1980, Science 210: 605–610.CrossRefGoogle Scholar
  25. Riggs, A., 1975, Cytogenet. Cell Genet. 14: 9–25.PubMedCrossRefGoogle Scholar
  26. Roberts, R., 1980, Gene 8: 329.PubMedCrossRefGoogle Scholar
  27. Robins, D. M., Axel, R., and Henderson, A. S., 1981, J. Mol. Appl. Genet. 1: 191–203PubMedGoogle Scholar
  28. Scangos, G. A., and Ruddle, F. H., 1981, Gene 14: 1–10.PubMedCrossRefGoogle Scholar
  29. Stein, R., Greenbaum, Y., Pollack, Y., Razin, A., and Cedar, H., 1982, Proc. Natl. Acad. Sci. U.S.A. 79: 61–65.PubMedCrossRefGoogle Scholar
  30. Stuhlmann, H., Jahner, D., and Jaenisch, R., 1981, Cell 26: 221–232.PubMedCrossRefGoogle Scholar
  31. Sutcliffe, J. G., 1978, Nucleic Acids Res. 5: 2721–2728.PubMedCrossRefGoogle Scholar
  32. Sutter, D., and Doerfler, W., 1980, Proc. Natl. Acad. Sci. U.S.A. 77: 253–256PubMedCrossRefGoogle Scholar
  33. Szybalska, E. H., and Szybalski, W., 1962, Proc. Natl. Acad. Sci. U.S.A. 48: 2026–2034.PubMedCrossRefGoogle Scholar
  34. Taylor, J. H., 1979, in: Molecular Genetics, Pt. 3, Chromosome Structure (J. H. Taylor, ed.), Academic Press, New York, pp. 89–115.Google Scholar
  35. Van der Ploeg, L. H. T., and Flavell, R. A., 1980, Cell 18: 947–958.CrossRefGoogle Scholar
  36. Vardimon, L., Neuman, R., Kuhlmann, I., Sutter, D., and Doerfler, W., 1980, Nucleic Acids Res. 8: 2461–2473.PubMedCrossRefGoogle Scholar
  37. Waechter, D. E., and Baserga, R., 1982, Proc. Natl. Acad. Sci. USA 79: 1106–1110PubMedCrossRefGoogle Scholar
  38. Wagner, M. J., Sharp, J. A., and Summers, W. C., 1981, Proc. Natl. Acad. Sci. U.S.A. 78: 1441–1445.PubMedCrossRefGoogle Scholar
  39. Wigler, M., Levy, D., and Perucho, M., 1981, Cell 24: 33–40.PubMedCrossRefGoogle Scholar
  40. Wyatt, G. R., 1951, Biochem. J. 48: 584–590.PubMedGoogle Scholar
  41. Yagi, M., and Koshland, M. E., 1981, Proc. Natl. Acad. Sci. U.S.A. 78: 4907–4911.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Barbara Christy
    • 1
  • George Scangos
    • 1
  1. 1.Department of BiologyThe Johns Hopkins UniversityBaltimoreUSA

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