Nucleotide Sequence and Structure Determination of Rabbit 18 S Ribosomal RNA
Ribosomes accomplish their translational role within a highly organized nucleoprotein structure. The specific aim for research on ribosomes, therefore, is to elucidate the architecture and eventually the function of these multi-molecular complexes. Prokaryotic 70 S ribosomes can be dissociated into a small 30 S subunit and a large 50 S subunit. The 30 S subunit contains 16 S ribosomal RNA (rRNA) in association with 21 proteins, while the 50 S subunit contains a 5 S rRNA and the 23 S rRNA combined with 31 proteins. Eukaryotic 80 S ribosomes are appreciably larger. Their small 40 S subunit is comprised of 18 S rRNA and approximately 30 proteins, while the large 60 S subunit is much more complex, with one molecule each of 5 S, 5.8 S, and 28 S rRNA and 45–50 proteins.
KeywordsPartial Digestion Cobra Venom Ribosomal Ribonucleic Acid Structure Buffer Enzymatic Probe
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