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Nucleotide Sequence and Structure Determination of Rabbit 18 S Ribosomal RNA

  • John F. Connaughton
  • Ajit Kumar
  • Raymond E. Lockard
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)

Abstract

Ribosomes accomplish their translational role within a highly organized nucleoprotein structure. The specific aim for research on ribosomes, therefore, is to elucidate the architecture and eventually the function of these multi-molecular complexes. Prokaryotic 70 S ribosomes can be dissociated into a small 30 S subunit and a large 50 S subunit. The 30 S subunit contains 16 S ribosomal RNA (rRNA) in association with 21 proteins, while the 50 S subunit contains a 5 S rRNA and the 23 S rRNA combined with 31 proteins. Eukaryotic 80 S ribosomes are appreciably larger. Their small 40 S subunit is comprised of 18 S rRNA and approximately 30 proteins, while the large 60 S subunit is much more complex, with one molecule each of 5 S, 5.8 S, and 28 S rRNA and 45–50 proteins.

Keywords

Partial Digestion Cobra Venom Ribosomal Ribonucleic Acid Structure Buffer Enzymatic Probe 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • John F. Connaughton
    • 1
  • Ajit Kumar
    • 1
  • Raymond E. Lockard
    • 1
  1. 1.Department of BiochemistryThe George Washington University School of MedicineUSA

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