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The Kallikrein-Like Activity Present in Human Euglobulin Fraction

  • W. Dias da Silva
  • Rocival L. Araujo
  • Maria N. R. Vieira
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 21)

Abstract

The euglobulin fraction was prepared essentially as described by Lepow et al. (1963). One volume of fresh human serum was adding to 8 volumes of pH 5. 5 acetate buffer, ionic strength 0. 02, the mixture was allowed to stand overnight and the precipitate was resuspended in 0. 5M NaC1 to one-tenth the original volume of serum. The preparation was centrifuged at 29. 000 RPM for 60 minutes, the particles of lipid were removed by filtration in glass-wool and the resultin opalescent solution containing the euglobulins was then dialyzed vs. pH 7. 5 phosphate buffer ionic strength 0.15. Human euglobulin prepared in this manner (C1 F1) contracts rat uterus only after a prolonged contact of 2 to 3 minutes (Fig. 1), fails to contract guinea-pig ileum, enhances vascular permeability in guinea-pig skin and forms kinin when incubated or either diluted human plasma or with partialy purified preparations of bradykininogen. The activity only appears after dialysis of the euglobulin preparation against pH 7. 5 phosphate buffer ionic strength 0. 15 (fig. 2).

Keywords

DEAE Cellulose Prolonged Contact DEAE Cellulose Chromatography KALlIKREIN Activity Fresh Human Serum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1972

Authors and Affiliations

  • W. Dias da Silva
    • 1
  • Rocival L. Araujo
    • 2
  • Maria N. R. Vieira
  1. 1.Department of BiochemistryInstituto de Ciências Biológicas da UFMGBrasil
  2. 2.Department of PhysiologyInstituto de Ciências Biológicas da UFMGBrasil

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