The Kallikrein-Like Activity Present in Human Euglobulin Fraction
The euglobulin fraction was prepared essentially as described by Lepow et al. (1963). One volume of fresh human serum was adding to 8 volumes of pH 5. 5 acetate buffer, ionic strength 0. 02, the mixture was allowed to stand overnight and the precipitate was resuspended in 0. 5M NaC1 to one-tenth the original volume of serum. The preparation was centrifuged at 29. 000 RPM for 60 minutes, the particles of lipid were removed by filtration in glass-wool and the resultin opalescent solution containing the euglobulins was then dialyzed vs. pH 7. 5 phosphate buffer ionic strength 0.15. Human euglobulin prepared in this manner (C1 F1) contracts rat uterus only after a prolonged contact of 2 to 3 minutes (Fig. 1), fails to contract guinea-pig ileum, enhances vascular permeability in guinea-pig skin and forms kinin when incubated or either diluted human plasma or with partialy purified preparations of bradykininogen. The activity only appears after dialysis of the euglobulin preparation against pH 7. 5 phosphate buffer ionic strength 0. 15 (fig. 2).
KeywordsDEAE Cellulose Prolonged Contact DEAE Cellulose Chromatography KALlIKREIN Activity Fresh Human Serum
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