Guinea Pig Prekallikrein Activator

  • Mary P. Treloar
  • Helen A. Pyle
  • Penelope J. Fuller
  • Henry Z. Movat
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 21)


Davies and Lowe (2,3) demonstrated that guinea pig serum contains a permeability factor distinct from anaphylatoxin after incubation with immune aggregates. It has also been shown that such treatment activates the kininforming system (5, 6). In the course of investigations into the components of the guinea pig intrinsic plasma kinin-forming system, a kinin-forming component has been differentiated from kallikrein by its inability to generate kinin from plasma heated to 61° for 60 minutes (8). This activator can be adsorbed to antigen-antibody precipitates and subsequently eluted (10). The isolated material possesses potent vascular permeability enhancing activity and the ability to contract the isolated rat uterus when incubated with fresh, non-glass contacted plasma. These properties have been used in the further isolation, purification and characterization of this material as an activator of plasma prekallikrein.


Polyacrylamide Electrophoresis Hageman Factor Protein Human Serum Albumin Sucrose Density Gradient Ultracentrifugation Immune Aggregate 


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Copyright information

© Plenum Press, New York 1972

Authors and Affiliations

  • Mary P. Treloar
    • 1
  • Helen A. Pyle
    • 1
  • Penelope J. Fuller
    • 1
  • Henry Z. Movat
    • 1
  1. 1.Division of Experimental Pathology and Institute of ImmunologyUniversity of TorontoTorontoCanada

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