Bradykininogen Synthesis by Liver
Kininogen disappears from peripheral blood after hepatectomy or intoxication with carbon tetrachloride (1), a point that suggests that kininogen is synthesized by the liver. In the present study we have tested this possibility by perfusing the isolated liver with Krebs-Henseleit solution containing 14C-arginine and 14C-proline. The radioactive protein formed by the isolated liver reacts with a specific kininogenase (2) to produce a radioactive peptide indistinguishable from bradykinin. We interpret our data to mean that kininogen is synthesized by the liver.
KeywordsLiver Perfusate Howard Hughes Medical Institute Hepatic Protein Synthesis Radioactive Protein Plasma Kininoge
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