Acid Dependent Kinin-Forming System in Mammalian Malignant and Normal Tissue
Previous studies have demonstrated that a mammalian tumor (Murphy-Sturm lymphosarcoma) contains the following components of a vasoactive kinin-forming system: kininogen, a kinin-destroying enzyme, and a pre-kallikrein proenzyme capable of being activated by acetone (1). Levels of kininogen increased during tumor development as did kininase activity. Acetone-precipitated tumor extracts formed kinin when incubated with heated (80°C rat) or human plasma. This kinin-forming activity initially increased during tumor growth and then diminished to almost zero levels within 12–14 days following tumor transplant. The kallikrein activity was similar to that of rat plasma kallikrein with respect to pH profile, kinetics of kinin formation (using human plasma kininogen as substrate at pH 7.8), and action of plant, mammalian, and synthetic protease inhibitors.
KeywordsAcid Proteinase Kinin Activity Tumor Extract Epsilon Amino Caproic Acid Bean Trypsin Inhibitor
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