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Simultaneous Measurement of Haemoglobin Oxygenation of Brain and Skeletal Muscle of Rat in Vivo by Near-Infrared Spectrophotometry

  • A. Seiyama
  • O. Hazeki
  • M. Tamura
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 215)

Abstract

Analysing transmitted light at 700 and 730 nm, we found a linear relationship between the blood concentration and the absorbance change in perfused hindlimb of rat when physiological haematocrits (Hc) in the range of 15 to 50% were used for the inflowing perfusate. Several optical parameters, which are required for calculation of changes in oxyhaemoglobin, deoxyhaemoglobin and blood volume, were determined. In the present paper, we report the application of a two-wavelength method to the simultaneous measurement of haemoglobin oxygenation of rat brain and skeletal muscle at steady state in vivo.

Keywords

Blood Volume Simultaneous Measurement Optical Parameter Absorbance Change Light Guide 
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References

  1. Ruderman, N.B., Houghton, C.R.S. and Hems, R. (1971). Evaluation of the isolated perfused rat hindquarter for the study of muscle metabolism. Biochem. J. 124, 639–651.Google Scholar
  2. Smith, J.J. and Kampine, J.P. (1980). Circulatory Physiology - The Essentials. The Williams & Wilkins Company, Baltimore, MD.Google Scholar
  3. Sugano, T., Suda, K., Shimada, M. and Oshino, N. (1978). Biochemical and ultrastructural evaluation of isolated rat liver systems perfused with hemoglobin-free medium. J. Biochem. (Tokyo), 83, 995–1007.Google Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • A. Seiyama
    • 1
  • O. Hazeki
    • 1
  • M. Tamura
    • 1
  1. 1.Research Institute of Applied ElectricityHokkaido University060 SapporoJapan

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