A Comparison of the Methods for Assessment of the Heterogeneity of Myocardial Capillary Spacing
In 1982, during the ISOTT Meeting in Dortmund, we presented a paper evaluating the methods for assessment of myocardial capillarity (Rakusan and Turek, 1984). In that paper we compared various morphometric methods for the estimation of the mean intercapillary distance and the heterogeneity of capillary spacing. The triangular method (Renkin et al., 1981) and the method of concentric circles (Loats, Sillau and Banchero, 1978) were used for obtaining the index of the heterogeneity. Both these methods are manual and hence, time consuming. Recently, we have computerized the closest-individual method introduced by Kayar et al. (1982) and have in addition developed our own method, the method of capillary domains (Hoofd et al., 1985). These two computerized methods are much faster and more convenient to handle than manual methods and therefore more attractive to use. However, before introducing the computerized methods, we had already evaluated some results with the old methods (Rakusan et al., 1984; Rakusan et al., 1986). Thus the question arose, whether the results obtained with the old manual methods and with the new computerized methods were comparable. Therefore we decided to compare the various methods through a critical evaluation of how they reflect experimentally-induced changes in the heterogeneity of the myocardial capillary spacing. In addition, we evaluated the methods with respect to their practicability.
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