Highly Polymerized Human Haemoglobin as an Oxygen-Carrying Blood Substitute

  • W. K. R. Barnikol
  • O. Burkhard
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 215)


There are many situations where a quick substitution of erythrocytes is warranted. However the need for blood typing generally causes considerable delay in getting the matched erythrocytes. Furthermore, in the case of a catastrophe or war there may be a great demand for blood which cannot be delivered by the blood donors available. In these cases an artificial oxygen-carrying blood substitute which does not require blood typing and which can be stored for a considerable time may save lives. For example, in the case of an accident such a blood substitute is available at once. Another field of application for such solutions lies in chronic peripheral arterial occlusion. It has been shown that in these cases an oxygen-carrying blood substitute is superior to simple isoncotic volume substitution (Moss et al., 1953).


Blue Dextran Blood Substitute Hemoglobin Solution Measure Residence Time Perfluorochemical Emulsion 
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  1. Barnikol, W.K.R. (1982a). Die Feinstruktur der Sauerstoff-HaemoglobinBindungskurve. Funkt. Biol. Med. 1, 46–52.Google Scholar
  2. Barnikol, W.K.R. (1982b). Der Einfluss der Effektoren auf die Feinstruktur der Sauerstoff-Haemoglobin-Bindungskurve. Funkt. Biol. Med. 1, 214–220.Google Scholar
  3. Barnikol, W.K.R. and Burkhard, O. (1983). Feinstruktur der Sauerstoff-Bindung und intermolekulare Wechselwirkung des Haemoglobins im Tierreich: Regenwurm, Frosch, Ente und Forelle. Funkt. Biol. Med. 2, 53–59.Google Scholar
  4. Burkhard, O. and Barnikol, W.K.R. (1983). Die Konzentrations-abhangigkeit molekularspezifischer physikalisch-chemischer und biologischer Eigenschaften des humanen Haemoglobins als Beweis fur intertetramere Wechselwirkungen. Funkt. Biol. Med. 2, 185–193.Google Scholar
  5. Clark, L.C., Jr and Gollan, F. (1966). Survival of mammals breathing organic liquids equilibrated with oxygen at atmospheric pressure. Science, 152, 1755–1756.CrossRefGoogle Scholar
  6. Gould, S.A., Rosen, A.L., Sehgal, L.R., Sehgal, H.L., Langdale, L.A., Krause, L.M., Rice, C.L., Chamberlin, W.H. and Moss, G.S. (1986). Fluosol-DA as a red cell substitute in acute anemia. N. Engl. J. Med. 314, 1653–1656.CrossRefGoogle Scholar
  7. Hirlinger, W.K., Grunert, A., Herrmann, M., Petutschnigk, D. andLanger, K. (1982). Auswirkungen eines teilweisen Blutaustausches mit Fluosol-DA 20% auf den intakten Organismus des Schweines. Anaesthesist, 31, 660–666.Google Scholar
  8. Hunt, C.A., Burnette, R.R., MacGregor, R.D., Strubbe, A.E., Lau, D.T., Taylor, N. and Kawada, H. (1985). Synthesis and evaluation of a prototypal artificial red cell. Science, 230, 1165–1168.CrossRefGoogle Scholar
  9. Kothe, N. and Bonhard, K. (1985). Characterization of a modified stroma-free hemoglobin solution as an oxygen-carrying plasma substitute. Surg. Gynecol. Obstet. 161, 563–569.Google Scholar
  10. Lowe, K.C. and Bollands, A.D. (1985). Physiological effects of perfluorocarbon blood substitutes. Med. Lab. Sci. 42, 367–375.Google Scholar
  11. Moss, G.S., Gould, S.A., Sehgal, L.R., Sehgal, H.L. and Rosen, A. (1984). Hemoglobin solution–from tetramer to polymer. In: The Red Cell, Sixth Ann Arbor Conference. Ed. Brewer, G., Alan R. Liss Inc., New York, pp. 191–207.Google Scholar
  12. Nicolau, Y.C. and Gersonde, K. (1977). Irreversible incorporation of allosteric effectors into lipid vesicles to improve unloading by hemoglobin in erythrocytes. Deutsches Patent 2, 740053.Google Scholar
  13. Pabst, R. (1977). Sauerstofftransport mit stromafreien Haemoglobin- losungen und Fluorocarbonen. Med. Klinik, 72, 1555–1562.Google Scholar
  14. Sharma, S.K., Bollands, A.D., Davis, S.S. and Lowe, K.C. (1987). Emulsified perfluorochemicals as physiological oxygen-transport fluids: assessment of a novel formulation. This volume.Google Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • W. K. R. Barnikol
    • 1
  • O. Burkhard
    • 2
  1. 1.Physiologisches InstitutJohannes Gutenberg-UniversitaetMainzGermany
  2. 2.Haematologische AbteilungJohannes Gutenberg-UniversitaetMainzGermany

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