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Oxygen Transport to Renal Tissue: Effect of Oxygen Carriers

  • G. Gronow
  • Th. Kelting
  • Ch. Skrezek
  • J. v.d. Plas
  • J. C. Bakker
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 215)

Abstract

The isolated perfused rat kidney introduced by Weiss and colleagues in 1959 has become a commonly used tool in the field of renal physiology and pharmacology (Weiss, Passow and Rothstein, 1959; Little and Cohen, 1974; Ross, 1978; Maack, 1986). In view of technical complications such as blood clotting and the release of vasoactive factors most authors preferred a hyperoxygenated (PO2~660 mmHg) balanced salt solution instead of blood as a perfusate. However, recent experiments with the isolated Ringer-perfused rat kidney indicate that oxygen transport to renal tissue has become a central question: due to a steep gradient of oxygen partial pressure in the outer medullary region of mammalian kidneys (Leichtweiss et al., 1969; Baumgartl et al., 1972) the poor oxygen binding capacity of hyperoxygenated salt solutions induced functional and morphological lesions in distinct renal tissue zones (Alcorn et al., 1981; Brezis et al., 1984; Schurek and Kriz, 1985). The aim of the present experiments was to compare the effects of three different oxygen carriers on function and tissue integrity of the isolated perfused rat kidney. The following served as oxygen carriers: a) coupled haemoglobin, b) washed erythrocytes, and c) perfluorocarbons. Perfusions performed with hyperoxygenated Ringer solutions (PO2~660 mmHg) served as a control. Our data indicate that renal function (perfusion flow rate, glomerular filtration rate, absolute and fractional Na+ reabsorption) as well as parameters of tissue integrity (i.e. the loss of enzymes, and tissue water content) are maintained best with an erythrocyte suspension and, for a limited time period, with coupled haemoglobin in the perfusate. They are reasonably maintained during Ringer perfusion, but are severely impaired in the presence of a perfluorocarbon emulsion.

Keywords

Oxygen Transport Oxygen Carrier Erythrocyte Suspension Blood Substitute Tissue Water Content 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • G. Gronow
    • 1
  • Th. Kelting
    • 1
  • Ch. Skrezek
    • 1
  • J. v.d. Plas
    • 2
  • J. C. Bakker
    • 2
  1. 1.Department of PhysiologyUniversity of KielKielGermany
  2. 2.Central LaboratoryNetherlands Red CrossAmsterdamThe Netherlands

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