Cytochrome P-450 and Vitamin E Free Radical Reductase: Formation of and Protection Against Free Radicals
In studies on lipid peroxidation, liver microsomes are frequently employed. Already in 1963, Hochstein and Ernstere1 reported that in their experiments lipid peroxidationin rat liver microsomes required molecular oxygen and NADPH and that lipid peroxidation intensified by the availability of ADP and Fe2+. This process is referred to as enzymic lipid peroxidation since it appears to depend on the presence of the haemoprotein cytochrome P-450 and the flavoprotein NADPH cytochrome P-450 reductase, both residing in the microsomal membrane2. The precise contribution of both proteins to NADPH dependent microsomal lipid peroxidation is still a matter of debate2. We described that cytochrome P-450 might be involved in both lipid hydroperoxide (LOOH) independent and LOOH dependent lipid peroxidation2.
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