In Vitro Oxidation of Low Density Lipoproteins

  • Martina Rotheneder
  • Georg Striegl
  • Hermann Esterbauer
Part of the NATO ASI Series book series (NSSA, volume 189)


Oxidized low density lipoproteins (o-LDL) are considered to be causally involved in the formation of early atherosclerotic leasons1,23 Lipid peroxidation is an extremely complex process and the consequences for LDL are manifold (for review see 4).


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  1. 1.
    U. P. Steinbrecher, S. Parthasaraty, D. S. Leake, J. L. Witztum and D. Steinberg, Modification of low density lipoprotein by endothelial cells involves lipid peroxidation and degradation of low density lipoprotein phospholipids, Proc. Natl. Acad. Sci. USA 81: 3883 (1984).CrossRefGoogle Scholar
  2. 2.
    J. W. Heinecke, Free radical modification of low density lipoprotein: Mechanisms and biological consequences. Free Radical Biology and Medicine 3: 65 (1987).CrossRefGoogle Scholar
  3. 3.
    G. Jürgens, H. F. Hoff, G. M. Chisolm III and H. Esterbauer, Modification of human serum low density lipoprotein by oxidation -characterization and pathophysiological implications, Chem. Phys. Lipids 45: 315 (1987).CrossRefGoogle Scholar
  4. 4.
    H. Esterbauer, O. Quehenberger and G. Jürgens, Oxidation of human low density lipoprotein with special attention to aldehydic lipid peroxidation products, in: “Free Radicals: Methodology and Concepts, C. Rice-Evans and B. Halliwell, eds., The Richelieu Press, London, 243 (1988).Google Scholar
  5. 5.
    H. Esterbauer, G. Striegl, H. Puhl and M. Rotheneder, Continuous monitoring of in vitro oxidation of human low density lipoprotein Free Rad. Res. Comms.: in press (1989).Google Scholar
  6. 6.
    M. E1-Saadani, H. Esterbauer, M. El-Sayed, M. Goher, A. Y. Nassar and G. Jürgens, A spectrophotometric assay for lipid peroxides in serum lipoproteins using a comercially available reagent, J. Lip. Res.: in press (1989).Google Scholar
  7. 7.
    K. H. Cheeseman, A. Beavis and H. Esterbauer, Hydroxyl-radicalinduced iron-catalysed degradation of 2-deoxyribose. Quantitative determination of malonaldehyde, Biochem. J. 252: 649 (1988).PubMedGoogle Scholar
  8. 8.
    M. E. Haberland, D. Fong and L. Cheng, Malondialdehyde-altered protein occurs in atheroma of Watanabe heritable hyperlipidemic rabbits, Science 241: 215 (1988).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Martina Rotheneder
    • 1
  • Georg Striegl
    • 1
  • Hermann Esterbauer
    • 1
  1. 1.Institute of BiochemistryUniversity of GrazGrazAustria

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