In Vitro Oxidation of Low Density Lipoproteins

  • Martina Rotheneder
  • Georg Striegl
  • Hermann Esterbauer
Part of the NATO ASI Series book series (NSSA, volume 189)


Oxidized low density lipoproteins (o-LDL) are considered to be causally involved in the formation of early atherosclerotic leasons1,23 Lipid peroxidation is an extremely complex process and the consequences for LDL are manifold (for review see 4).


Lipid Hydroperoxide Lipid Peroxidation Process Tertiary Reaction Aldehydic Lipid Peroxidation Product Density Lipoprotein Phospholipid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    U. P. Steinbrecher, S. Parthasaraty, D. S. Leake, J. L. Witztum and D. Steinberg, Modification of low density lipoprotein by endothelial cells involves lipid peroxidation and degradation of low density lipoprotein phospholipids, Proc. Natl. Acad. Sci. USA 81: 3883 (1984).PubMedCrossRefGoogle Scholar
  2. 2.
    J. W. Heinecke, Free radical modification of low density lipoprotein: Mechanisms and biological consequences. Free Radical Biology and Medicine 3: 65 (1987).PubMedCrossRefGoogle Scholar
  3. 3.
    G. Jürgens, H. F. Hoff, G. M. Chisolm III and H. Esterbauer, Modification of human serum low density lipoprotein by oxidation -characterization and pathophysiological implications, Chem. Phys. Lipids 45: 315 (1987).PubMedCrossRefGoogle Scholar
  4. 4.
    H. Esterbauer, O. Quehenberger and G. Jürgens, Oxidation of human low density lipoprotein with special attention to aldehydic lipid peroxidation products, in: “Free Radicals: Methodology and Concepts, C. Rice-Evans and B. Halliwell, eds., The Richelieu Press, London, 243 (1988).Google Scholar
  5. 5.
    H. Esterbauer, G. Striegl, H. Puhl and M. Rotheneder, Continuous monitoring of in vitro oxidation of human low density lipoprotein Free Rad. Res. Comms.: in press (1989).Google Scholar
  6. 6.
    M. E1-Saadani, H. Esterbauer, M. El-Sayed, M. Goher, A. Y. Nassar and G. Jürgens, A spectrophotometric assay for lipid peroxides in serum lipoproteins using a comercially available reagent, J. Lip. Res.: in press (1989).Google Scholar
  7. 7.
    K. H. Cheeseman, A. Beavis and H. Esterbauer, Hydroxyl-radicalinduced iron-catalysed degradation of 2-deoxyribose. Quantitative determination of malonaldehyde, Biochem. J. 252: 649 (1988).Google Scholar
  8. 8.
    M. E. Haberland, D. Fong and L. Cheng, Malondialdehyde-altered protein occurs in atheroma of Watanabe heritable hyperlipidemic rabbits, Science 241: 215 (1988).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Martina Rotheneder
    • 1
  • Georg Striegl
    • 1
  • Hermann Esterbauer
    • 1
  1. 1.Institute of BiochemistryUniversity of GrazGrazAustria

Personalised recommendations