Pentane Measurement, an Index of in Vivo Lipoperoxidation: Applications and Limits of the Method

  • J. Pincemail
  • C. Deby
  • M. Lismonde
  • Y. Bertrand
  • G. Camus
  • M. Lamy
Part of the NATO ASI Series book series (NSSA, volume 189)


Lipid peroxidation induced by free radicals generation has been implicated in several pathological conditions including adult respiratory distress syndrome1 and cancer2, in cardiac surgery such as cardiopulmonary bypass3, in the ageing process4, in the ischemic-reperfusion phenomenon5, etc...;


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    M. Lamy, G. Deby-Dupont, J. Pincemail, M. Braun, J. Duchateau, C. Deby, J. Van Erck, L. Bodson, P. Damas and P. Franchimont. Biochemical pathways of acute lung injury. Bull. Eur. Physiopathol. Respir. 21: 221 (1985)Google Scholar
  2. 2.
    B. N. Ames, Dietary carcinogens and anticarcinogens. Oxygen radicals and degenerative disease Science 221: 1256 (1983)PubMedGoogle Scholar
  3. 3.
    N. C. Cavarocchi, M. D. England, J. F. O’Brien, E. Solis, P. Russo, H. V. Schaff, T. A. Orszulak, J R. Pluth and M. P. Kaye, Superoxide formation during cardiopulmonary bypass. Is there a role for vitamin E, J. Surg Res 40: 519 (1986).CrossRefGoogle Scholar
  4. 4.
    D. Harman, Free radical theory of aging: role of free radicals in the origination and evolution of life, aging, and disease processes in “Free radicals, aging and degenerative disease”. eds, J.E. Johnson, R. Walford, D. Hannan and J. Miguel; Alan R. Liss, Inc, New York, pp 3 (1986).Google Scholar
  5. 5.
    R. Bolli, B. S. Patel, M. O. Jeroudi, E. K. Lai and P. B McCay, Demonstration of free radical generation in “stunned” myocardium of intact dogs with the use of the spin trap -phenyl N-tert-butyl nitrone, J, Clin. Invest. 82: 476 (1988)CrossRefGoogle Scholar
  6. 6.
    T. Asakawa and S. Matsushita, Thiobarbituric acid test for detecting lipid peroxides, Lipids 14: 401 (1979)CrossRefGoogle Scholar
  7. 7.
    M. Hamberg, J. Svensson, B. Samuelsson, Prostaglandin endoperoxides. A new concept concerning the mode of action and release of prostaglandins, Proc Natl Acad Sci USA 71: 3284 (1974)Google Scholar
  8. 8.
    R. O. Recknagel and E. A Glende Jr, Spectrophotometric detection of lipid conjugated dienes Methods in Enzymology 105: 331 (1984)PubMedGoogle Scholar
  9. 9.
    H. Esterbauer, K. H. Cheeseman, M. U. Dianzani, G. Poli and T. F Slater, Separation and characterization of the aldehydic products of lipid peroxidation stimulated by ADF-Fe2+ in rat liver microsomes, Biochem J 208: 129 (1982)CrossRefGoogle Scholar
  10. 10.
    C. D. Evans, G. R. List, A. Dolev, D. G. Connell and R. L. Hoffman, Pentane from thermal decomposition of lipoperoxidase-derived products, Lipids 2: 432 (1967)CrossRefGoogle Scholar
  11. 11.
    C. Deby, J. Pincemail, Y. Bertrand, M. Lismonde, M. Lamy and R.Goutier, Consumption of pentane by hepatic microsomes and consequences on pentane measurement in exhaled gases, Arch Int Physio.Biochim. 94: S19 (1986)Google Scholar
  12. 12.
    C. R. Wade and A. M. van Rij, In vivo lipid peroxidation in man. measured by the respiratory excretion of ethane, pentane and other lowmolecular-weight-hydrocarbons, Anal. Biochem. 150:1 (1985)Google Scholar
  13. 13.
    M. Sagai and T. Ichinose, Age-related changes in lipid peroxidation as measured by ethane, ethylene, butane and pentane in respired gases of rats, Life Sciences 27: 731 (1980)CrossRefGoogle Scholar
  14. 14.
    C. J. Dillard, E. E. Dumelin and A. L. Tappel, Effect of dietary vitamin E on expiration of pentane and ethane by the rat, Lipids 12: 109 (1977).CrossRefGoogle Scholar
  15. 15.
    C. J. Dillard, R. E. Litov and A. L. Tappel, Effects of dietary vitamin E, selenium, and polyunsaturated fats on in vivo lipid peroxidation in the rat as measured by pentane production, Lipids 13: 396 (1978)CrossRefGoogle Scholar
  16. 16.
    D. G. Hafeman and W. G. Hoekstra, Lipid peroxidation in vivo during vitamin E and selenium deficiency in the rat as monitored by ethane evolution, J. Nutr. 107: 666 (1977)CrossRefGoogle Scholar
  17. 17.
    U. Mister, D. Albrecht and H. Kappus, Evidence for carbon tetrachloride-and ethanol-induced lipid peroxidation in vivo demonstrated by ethane production in mice and rats, Toxicol. Appl. Pharmacol. 41: 639 (1977)CrossRefGoogle Scholar
  18. 18.
    M. Sagai and A. L. Tappel, Lipid peroxidation induced by some halomethanes as measured by in vivo pentane production in the rat, Toxicol. Appl. Pharmacol. 49: 283 (1979)CrossRefGoogle Scholar
  19. 19.
    J. G. Miser, H. M. Bolt, H. Muliawan and H. Kappus, Quantitative evaluation of ethane and n-pentane as indicators of lipid peroxidation in vivo, Arch. Toxicol. 52: 135 (1983)Google Scholar
  20. 20.
    C. J. Dillard, J. E. Downey and A.L. Tappel, Effects of antioxidant on lipid peroxidation in iron-loaded rats, Lipids 19: 127 (1984)CrossRefGoogle Scholar
  21. 21.
    G. Cohen, Lipid peroxidation: detection in vivo and in vitro through the formation of saturated hydrocarbon gases, in “Oxygen free radicals and tissue damage” Ciba Found Symp 65 Excerta Medica Amsterdam, Oxford, New York, pp 177 (1979)Google Scholar
  22. 22.
    R. E. Litov, D. L. Gee, J. E. Downey and A. L. Tappel, The role of lipid peroxidation during chronic and acute exposure to ethanol as determined by pentane expiration in the rat, Lipids 16: 52 (1981)CrossRefGoogle Scholar
  23. 23.
    E. E. Dumelin, C. J. Dillard and A. L. Tappel, Effects of vitamin E and ozone on pentane and ethane expired by rats, Arch. Environ, Health 33: 129 (1978)Google Scholar
  24. 24.
    M. P. Habib, C. Eskelson and M. A. Katz, Ethane production rate in rats exposed to high oxygen concentration, Am. Rev. Respir. Dis. 137: 341 (1988)CrossRefGoogle Scholar
  25. 25.
    F. O. O’Neal, D. B. Menzel and M. D. Karis, Pentane expiration: a measure of halothane-induced peroxidation, Anesthesiology 51: S255 (1979)CrossRefGoogle Scholar
  26. 26.
    C. J. Dillard, R. E. Litov, W. M. Savin, E. E. Dumelin and A. L. Tappel, Effects of exercise, vitamin E, and ozone on pulmonary function and lipid peroxidation. J. Appl. Physiol.: Respirat, Environ. Exercise Physiol. 45 (6): 927 (1978)CrossRefGoogle Scholar
  27. 27.
    S. Morita, M. T. Snider, Y. Inada, Increased N-pentane excretion in humans: a consequence of pulmonary oxygen exposure, Anesthesiology 64: 730 (1986).CrossRefGoogle Scholar
  28. 28.
    J. R. Wispe, E. F. Bell and R. J. Roberts, Assessment of lipid peroxidation in newborn infants and rabbits by measurements of expired ethane and pentane: influence of parenteral lipid infusion, Pediat. Res. 19: 374 (1985)Google Scholar
  29. 29.
    M. Lemoyne, A. Van Gossum, R. Kurian, M. Ostro, J. Axler, K. N. Jeejeebhoy, Breath pentane analysis as an index of lipid peroxidation: a functional test of vitamin E status, Am. J. Clin. Nutr. 46: 267 (1987)CrossRefGoogle Scholar
  30. 30.
    K.F. Heim, U. M. Makila, R. Leveson, G. S. Ledley, G. Thomas, C. Rackley and P. W. Ramwell, Detection of pentane as a measurement of lipid peroxidation in humans using gas chromatography with a photoionization detector, in: “Lipid Mediators in the Immunology of Shock”, M. Paubert-Braquet, ed., NATO ASI series, Plenum Press, New York and London, pp 103 (1987)Google Scholar
  31. 31.
    M. T. Snider, P. O. Balke, K. E. Oerter, N. A. Francalancia, K. A. Pasko, M. E. Robbins, G. S. Gerhard and R. P. Richard, Methods for measuring lipid peroxidation products in the breath of man, Life Chem. Rep. 3: 168 (1985)Google Scholar
  32. 32.
    J. Pincemail, C. Deby, A. Dethier, Y. Bertrand, M. Lismonde, and M. Lamy, Pentane measurement in man as an index of lipoperoxidation, Bioelec. and Bioenerg. 18: 117 (1987)CrossRefGoogle Scholar
  33. 33.
    C. Deby, J. Pincemail, Y. Bertrand and M. Lismonde, The significance of pentane measurements in man, in: “Lipid Mediators in the Immunologof Shock”, M. Paubert-Braquet, ed., NATO ASI series, Plenum Press, New York and London, pp 97 (1987)Google Scholar
  34. 34.
    K. J. A. Davies, A. T. Quintanilha, G. A Brooks and L. Packer, Free radicals and tissue damage produced by excercise, Biochem. Biophys. Res Comm. 107 (4): 1198 (1982)CrossRefGoogle Scholar
  35. 35.
    P. B. McCay and M. M. King, Vitamin E: its role as biological free radical scavenger and its relationship to the mixed-function oxidase system in: “ Vitamin E: a comprehensive treatise”, eds, L.J. Machlin, New York, Marcel Dekker Inc, pp 289 (1980)Google Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • J. Pincemail
    • 1
  • C. Deby
    • 1
  • M. Lismonde
    • 2
  • Y. Bertrand
    • 3
  • G. Camus
    • 4
  • M. Lamy
    • 2
  1. 1.Laboratoire de Biochimie et RadiobiologieUniversité de Liège, Institut ChimieLiège IBelgium
  2. 2.Service d’AnesthésiologieCHULiège IBelgium
  3. 3.Clinique Saint-JeanBruxellesBelgium
  4. 4.lnstitut Supérieur d’Education physiqueLiège IBelgium

Personalised recommendations