Measurement of Superoxide and Superoxide Dismutase by Electron Spin Resonance and Chemiluminescence Assay
Spin trapping using dimethyl-1-pyrroline-N-oxide (DMPO) s a spin trapper is a useful method for the detection of superoxidel, 2. DMPOO0H which is formed by the reaction of DMPO and superoxide gives a characteristic electron spin resonance (ESR) signal. This combined with its superoxide dismutase (SOD) inhibitability, is making it possible to give a two fold garantee of specificity to this assay. Because of the relatively short life time of DMPO-OOH, to exactly quantify superoxide is difficult. However it is still possible to semi-quantify in cases where the rate of superoxide generation is relatively constant3. These methods may be advantageous when the materials show a strong light absorption or turbidity.
KeywordsElectron Spin Resonance Electron Spin Resonance Spectrum Xanthine Oxidase Polymorphonuclear Leukocyte Electron Spin Resonance Spectrometer
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