Methods of Measuring Lipid Peroxidation in Biological Systems: An Overview

  • Kevin H. Cheeseman
Part of the NATO ASI Series book series (NSSA, volume 189)


Lipid peroxidation is an extremely complicated process, especially in biological systems where one is dealing with mixtures of different lipids in matrices also containing proteins, carbohydrates, nucleic acids and catalytically-active enzymes and trace metals. With regard to measuring lipid peroxidation, the complexity of the process may be viewed by an optimist as presenting the investigator with a large number of opportunities: it is a multi-stage process and each stage can be investigated by at least one technique. The view of the pessimist, or perhaps realist, would be that the complexity of lipid peroxidation is a problem, in that no single assay method can fully describe what is happening or has happened in a system undergoing lipid peroxidation. In considering the large range of assay methods concerned with measuring lipid peroxidation, it is readily obvious that some are straightforward to perform, others tedious; some are more sensitive than others, some more prone to interference; some measure end-products, some measure unstable intermediates, and some measure specific identified compounds whilst others measure poorly characterised entities.


Lipid Peroxidation Lipid Hydroperoxide Electron Spin Resonance Spectroscopy Diene Conjugation Aldehydic Product 


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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Kevin H. Cheeseman
    • 1
  1. 1.Department of Biology and BiochemistryBrunel UniversityUxbridgeEngland

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