Biological Applications of Time-Gated Fluorescence Spectroscopy
The diagnostic use of fluorescence emission from both endogenous and exogenous fluorophores has found wide application in biology and medicine. At present in most cases fluorescence diagnosis is based on continuous wave (cw) measurements. This technique is applicable when the fluorescent emission from the target is sufficiently intense to be detected, and can be separated from the background through an appropriate selection of either excitation or emission wavelength. However in many cases quenching mechanisms strongly reduce the fluorescence, or the spectral overlapping of different fluorophors does not allow effective discrimination or localization of a target exogenous dye.
KeywordsUptake Time CTAB Concentration Gated Spectrum Amplitude Converter Single Photon Counting Method
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- 1.J. R. Lakowicz, “Principles of Fluorescence Spectroscopy”, Plenum Press, New York (1983)Google Scholar
- 10.D. V. O’Connor and D. Phillips, “Time-correlated single photon counting”, Academic Press, London, New York, Ch. 7, pp. 211–251 (1984)Google Scholar
- 12.R. L. Lipson, E. J. Baldes and A. M. Olsen, “The use of a derivative of hematoporhyrin in tumor detection”, J. Natl. Cancer Inst., vol. 26, pp. 1–8 (1961)Google Scholar
- 16.R. Redmond, E. J. Land and T. G. Truscott, “A comparison of the photo-physical properties of porphyrins used in cancer phototherapy”, in: “Primary Photoprocesses in Biology and Medicine”, edited by R. V. Bensasson, G. Jori, E. J. Land and T. G. Truscott, Plenum Press, New York, pp. 335–339 (1985)Google Scholar