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Membrane Topology of Cytochromes P-450: Elements and Measurement by Spectroscopic Techniques

  • A. Stier
  • V. Krüger
  • T. Eisbein
  • S. A. E. Finch
Part of the NATO ASI Series Advanced Science Institutes Series book series (NSSA, volume 202)

Abstract

The main concern of these lectures is: Do liver microsomal cytochromes P-450 have an oligomeric structure and is cooperativity involved in their function? Elements of topology of membrane proteins and the spectroscopic techniques used to investigate this toplogy are described. Oligomeric structure is an important element of membrane topology and information on it can be obtained from measurements of rotational diffusion of the proteins. Emphasis is therefore laid on photoselection techniques (time-dependent polarized delayed fluorescence and phosphorescence and time-dependent absorption dichroism).

Keywords

Rotational Diffusion Transition Moment Rotational Correlation Time Membrane Topology Anisotropy Decay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • A. Stier
    • 1
  • V. Krüger
    • 1
  • T. Eisbein
    • 1
  • S. A. E. Finch
    • 1
  1. 1.Max-Planck-Institut für biophysikalische ChemieAbteilung SpektroskopieGöttingenGermany

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