Early Events in the Formation of the Vertebrate Brain

  • Linda S. Ross
  • Stephen S. EasterJr.
Part of the NATO ASI Series book series (NSSA, volume 192)


For many years Dr. Easter’s research has focused on the development of the retinotectal pathway in fish and amphibians. Because these animals grow throughout life and continue to add new cells to the retina, they are more accessible and easier to examine than embryos. Dr. Easter’s previous investigations have suggested that retinal axons added late in life probably use the optic fibers that preceded them as guides to the optic tectum. In an attempt to understand the origin of the first axons, Dr. Easter and his colleagues have examined embryonic neural development, and that work is described here. This chapter first covers work done in collaboration with Dr. Jeremy Taylor in the laboratory of Dr. Michael Gaze on the early development of the optic pathway and its microenvironment in Xenopus laevis (Easter and Taylor, 1989). Second, the early neuronal scaffolding in the zebrafish brain, work done in collaboration with Drs. Stephen Wilson and Linda Ross and Mr. Timothy Parrett (Wilson et al., 1990), is described.


Growth Cone Anterior Commissure Optic Tectum Xenopus Embryo Posterior Commissure 
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  1. Bastiani, M.J., du Lac, S., and Goodman, C.S., 1985, The first neuronal growth cones in insect embryos. Model system for studying he development of neuronal specificity, in “Model Neural Networks and Behavior”, A.I. Seiverston, ed., Plenum Press, New York, p. 149.Google Scholar
  2. Constantine-Paton, M. and Capranica, R.R., 1976, Axonal guidance of transplanted eye primordia. J. Comp. Neurol., 170:17.PubMedCrossRefGoogle Scholar
  3. Easter, S.S., 1987, Retinal axons and the basal lamina, in: “Mesenchymal-Epithelial Interactions in Neural Development,” J.R. Wolff, J. Sievers, and M. Berry, eds., Springer-Verlag, Berlin , p. 385.Google Scholar
  4. Easter, S.S. and Taylor, J.S.H., 1989, The development of the Xenopus retinofugal pathway: optic fibers join a pre-existing tract, Development (in press).Google Scholar
  5. Easter, S.S., Schmidt, J.T., and Leber, S.M., 1978, The paths and destinations of the induced ipsilateral retinal projection in goldfish, J. Embryol Exp. Morph., 45:145.PubMedGoogle Scholar
  6. Gaze, R.M. and Fawcett, J.W., 1983, Pathways of Xenopus optic fibres regenerating from normal and compound eyes under various conditions, J. Embryol. Exp. Morph., 73:17.PubMedGoogle Scholar
  7. Harrelson, A.L. and Goodman, C.S., 1988, Growth cone guidance in insects: Fasciclin II is a member of the irnmunoglobulin superfamily, Science, 242:700.PubMedCrossRefGoogle Scholar
  8. Harris, W.A., 1986, Homing behavior of axons in the embryonic vertebrate brain, Nature, 320:266.PubMedCrossRefGoogle Scholar
  9. Harris, W.A., 1989, Local positional cues in the neuroepithelium guide retinal axons in embryonic Xenopus bruin, Nature, 339:218.CrossRefGoogle Scholar
  10. Harris, W.A., Holt, C.E., and Bonhoeffer, F., 1987, Retinal axons with Xenopus embryos: a time-lapse video study of single fibre in vivo, Development, 101:123.PubMedGoogle Scholar
  11. Herrick, C.J., 1938, Development of the cerebrum of Amblystoma during the early swimming stages, J. Comp. Neurol, 68:203.CrossRefGoogle Scholar
  12. Katz, M.J. and Lasek, R.J., 1978, Eyes transplanted to tadpole tails send axons rostrally in two spinal cord tracts, Science, 109:202.CrossRefGoogle Scholar
  13. Katz, M.J. and Lasek, R.J., 1979, Substrate pathways which guide growing axons in Xenopus embryos, J. Comp. Neurol., 183:817.PubMedCrossRefGoogle Scholar
  14. Kuwada, J., 1986, Cell recognition by neuronal growth cones in a simple vertebrate embryo, Science, 233:740.PubMedCrossRefGoogle Scholar
  15. Layer, P.G., 1983, Comparative localization of acetylcholinesterase and pseudoCholinesterase during morphogenesis of the chicken brain, Proc. Natl. Acad. Sci. USA, 80:6413.PubMedCrossRefGoogle Scholar
  16. Layer, P.G., Rommel, S., Bulthoff, H., and Hengstenberg, R., 1988, Independent spatial waves of biochemical differentiation along the surface of chicken brain as revealed by the sequential expression of acetylcholinesterase, Cell Tissue Res., 251:587.PubMedCrossRefGoogle Scholar
  17. Moody, S.A. and Stein, D.B., 1988, The development of acetylcholinesterase activity in the embryonic nervous system of the frog, Xenopus laevis, Dev. Brain Res., 39:225.CrossRefGoogle Scholar
  18. Nordlander, R.N., 1984, Developing descending neurons of the early Xenopus tail spinal cord in the caudal spinal cord of early Xenopus, J. Comp. Neurol., 228:117.CrossRefGoogle Scholar
  19. Nordlander, R.H., and Singer, M., 1982a, Spaces precede axons in the embryonic spinal cord, Exp. Neurol., 75:221.PubMedCrossRefGoogle Scholar
  20. Nordlander, R.H. and Singer, M., 1982b, Morphology and position of growth cones in the developing Xenopus spinal cord, Dev. Brain Res., 4:181.CrossRefGoogle Scholar
  21. Silver, J., and Rutishauser, U., 1984, Guidance of optic axons by a preformed adhesive pathway on neuroepithelial endfeet, Dev. Biol., 106:485.PubMedCrossRefGoogle Scholar
  22. Singer, M., Nordlander, R.H., and Egar, M., 1979, Axonal guidance during embryogenesis and regeneration in the spinal cord of the newt: the blueprint hypothesis of neuronal pathway patterning, J. Comp. Neurol., 188:1.CrossRefGoogle Scholar
  23. Steedman, J.G., Stirling, R.V., and Gaze, R.M., 1979, The central pathways of optic fibres in Xenopus tadpoles, J. Embryol. Exp. Morph., 50:199.PubMedGoogle Scholar
  24. Stuermer, C.A.O., 1988, Retinotopic organization of the developing retinotectal projection in the zebrafish embryo, J. Neurosci., 8:4513.PubMedGoogle Scholar
  25. Weiss, P., 1941, Nerve patterns: mechanics of growth, Growth, 5:163.Google Scholar
  26. Wilson, S.W., Ross, L.S., Parrett, T., and Easter, S.S.Jr., 1990, The development of a simple scaffold of axon tracts in the brain of the embryonic zebrafish, Brachydanio rerio, Development (in press).Google Scholar
  27. Wolfgang, W.J., and Forte, M.A., 1989, Expression of acetylcholinesterase during visual system development in Drosophila, Dev. Biol, 131:321.Google Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Linda S. Ross
    • 1
  • Stephen S. EasterJr.
  1. 1.Department of BiologyUniversity of MichiganAnn ArborUSA

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