In Vitro Investigations of Stone Growth Inhibition and Dissolution
Using the stone forming simulator1, we treated sections of whewellite stones with various urines. It was necessary to prevent bacterial contamination and growth which might otherwise alter the pH of the system. This could be controlled by adding a disinfectant (Betaisodona). Into each 4 filter crucibles two parts of a whewellite stone were suspended. The weight of the stone fragments was measured directly using microscales. Each was bathed with urine of various compositions. During the experiment the weight of the stones and the pH of the bathing solution were measured every 12 h. Since a new 24-h urine was collected daily samples were taken before, at 12 h and at 24 h for the analysis of Na, K, Ca, Mg and Cl. At the end of the experiment the two fragments of the whewellite stone were dried. One was used for scanning electron microscopy (SEM) and energy dispersive element analysis and the other for X-ray diffraction analysis.
KeywordsUric Acid Scanning Electron Microscopy Picture Apatite Crystal Daily Sample Scanning Electron Microscopy Investigation
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- 1.H. P. Bastian and M. A. H. Gebhardt, Akt. Urol. 7:275 (1976).Google Scholar