Combined Enzymatic Degradation with Chondroitinases and Alcian Blue Precipitation in Determination of Urinary Chondroitin Sulphates
It has been claimed that glycosaminoglycans are important inhibitors of calcium oxalate crystal growth and aggregation1. The mode of action as inhibitors may be through a binding of the glycosaminoglycans to the crystal surface and thereby blocking the growth sites of the crystals2. Chondroitin sulphates can also be recovered from calcium oxalate crystals after dissolution of crystals grown in urine3. The alcian blue precipitation method4is not specific for urinary glycosaminoglycans, but also precipitates polyanionic urinary glycoproteins and possibly ribonucleic acid residues. Using this method it has been shown that stone formers have a lower urinary excretion of polyanions, that are precipitable by alcian blue5. The purpose of the present study was to measure glycosaminoglycans specifically in unconcentrated urine.
KeywordsUrinary Excretion Heparan Sulphate Chondroitin Sulphate Alcian Blue High Excretion
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- 2.B. Fellström, B. G. Danielson, F. A. Karlsson, and S. Ljunghall, in: “Urolithiasis and Related Clinical Research”, P. O. Schwille, L. H. Smith, W. G. Robertson, and W. Vahlensieck, eds., Plenum, New York (1985).Google Scholar
- 3.J. G. Brockis, R. C. Bowyer, and R. K. McCulloch, Scand. J. Urol. Nephrol. Suppl. 53:67 (1979).Google Scholar