The Measurement of Oxalate and Glycolate with Immobilized Enzyme Systems
Renal stone disease afflicts up to 10% of the male population and calcium oxalate is a major component in over 70% of these stones1,2. Diet has traditionally been considered to be the main source of oxalate but, more recently, it has become accepted that the majority of oxalate is derived from endogenous metabolism. Glycolic acid has been identified as a major precursor of oxalate2,3 and variations in the urinary excretion of this precursor may be important. However, there is little information available regarding its excretion. For the study of the effects of glycolate and oxalate excretion on renal stone formation, specific and sensitive assays for these compounds have been developed in our laboratory.
KeywordsImmobilize Enzyme Ammonium Sulphate Calcium Oxalate Glycolic Acid Oxalate Oxidase
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- 2.A. Hodgkinson, “Oxalic Acid in Biology and Medicine”, Academic Press, New York (1977).Google Scholar