Determination of Urinary Oxalate by Reversed-Phase ION-Pair “High-Performance” Liquid Chromatography
Calcium oxalate is an important constituent of the majority of renal stones. Thus measurement of urinary oxalate has become essential in the basic evaluation of the urolithiasis patient. However, the large number of published methods indicate the presence of considerable methodological difficulties1. In this paper we describe our recently developed method for determination of urinary oxalate, based on a reversed-phase ion-pair “high-performance” liquid chromatography (HPLC)2. We also describe a comparison performed between our HPLC method, the colorimetric method of Hodgkinson and Williams3, and a commercial enzymatic method4.
KeywordsColorimetric Method HPLC Method Enzymatic Method Calcium Oxalate Renal Stone
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