Enzymatic Assay of Oxalate Using Oxalate Oxidase from Sorghum Leaves

  • C. S. Pundir
  • R. Nath
  • S. K. Thind


Estimation of oxalate in biological fluids using plant and moss enzyme has met with limited success owing to the inhibition of the enzyme activity by sodium ions normally present in these fluids1–4. In this communication, we report on the separation and partial purification of a highly active oxalate oxidase from sorghum leaves which is not inhibited by sodium ions in physiological concentrations and is suitable for direct estimation of oxalate in urine and other biological fluids.


Biological Fluid Sodium Succinate Urinary Oxalate Oxalate Concentration Oxalate Oxidase 
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  1. 1.
    J. Chiriboga, Biochem. Biophys. Res. Commun. 11:277 (1963).CrossRefGoogle Scholar
  2. 2.
    J. Chiriboga, Arch. Biochem. Biophys. 116:516 (1966).PubMedCrossRefGoogle Scholar
  3. 3.
    M. J. Laker, A. F. Hofman, and B. J. D. Meeuse, Clin. Chem. 26:827 (1980).PubMedGoogle Scholar
  4. 4.
    J. E. Buttery, N. Ludvigsen, E. A. Braiotta, and P. R. Pannal, Clin. Chem. 29:700 (1983).PubMedGoogle Scholar
  5. 5.
    C. S. Pundir and R. Nath, Phytochemistry (1984) (in press).Google Scholar
  6. 6.
    R. Bais, N. Potenzy, J. B. Edward, A. M. Rofe, and R. A. J. Conyers, Analyt. Chem. 52:508 (1980).CrossRefGoogle Scholar
  7. 7.
    S. K. Shrivastava and P. S. Krishnan, Biochem. J. 85:33 (1962).Google Scholar

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • C. S. Pundir
    • 1
  • R. Nath
    • 1
  • S. K. Thind
    • 1
  1. 1.Dept. of BiochemistryPostgraduate Institute of Medical Education and ResearchChandigarhIndia

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