The Effect of Ascorbic Acid on Urine Oxalate Measurement
We report that conditions employed in the pre-treatment of the urine are crucial to the correct estimation of oxalate. Interference of ascorbic acid, a common urinary constituent, has been noted in a number of different methods. After systematic investigation of the steps in our assay, we have found that this interference is associated with the pre-treatment conditions, particularly any pH adjustment with alkali, pH of precipitation, and collection and storage of urine. We describe conditions for assay which give minimal interference by ascorbic acid and which are applicable to all urinary oxalate methods.
KeywordsAscorbic Acid Urine Collection Correct Estimation Minimal Increase Minimal Interference
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