Radioimmunoassay of Vasoactive Intestinal Polypeptide (VIP) in Plasma
A sensitive and specific radioimmunoassay for VIP has been developed which can detect 3.3 pmol liter-1 of the peptide in plasma1. Antisera to highly purified porcine VIP covalently coupled to bovine serum albumin by the use of carbodiimide were raised in eight rabbits. The immunization dose was 15 nmol (50 μg) VIP per rabbit and injections were given subcutaneously at 8 week1 intervals. The final dilution, the avidity, and the specificity of each antiserum were determined.
KeywordsVasoactive Intestinal Polypeptide Vagal Nerve Pancreatic Polypeptide Final Dilution Specific Radioimmunoassay
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