Radioimmunoassay of Vasoactive Intestinal Polypeptide (VIP) in Plasma

  • J. Fahrenkrug
  • O. B. Schaffalitzky De Muckadell
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 106)


A sensitive and specific radioimmunoassay for VIP has been developed which can detect 3.3 pmol liter-1 of the peptide in plasma1. Antisera to highly purified porcine VIP covalently coupled to bovine serum albumin by the use of carbodiimide were raised in eight rabbits. The immunization dose was 15 nmol (50 μg) VIP per rabbit and injections were given subcutaneously at 8 week1 intervals. The final dilution, the avidity, and the specificity of each antiserum were determined.


Vasoactive Intestinal Polypeptide Vagal Nerve Pancreatic Polypeptide Final Dilution Specific Radioimmunoassay 
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  1. 1.
    Fahrenkrug J, Schaffalitzky de Muckadell OB: Radioimmunoassay of vasoactive intestinal polypeptide (VIP) in plasma. J. Lab. Clin. Med. 89, 1379–1388, 1977PubMedGoogle Scholar
  2. 2.
    Schaffaltizky de Muckadell OB, Fahrenkrug J, Holst JJ, Lauritsen KB: Release of vasoactive intestinal polypeptide (VIP) by intraduodenal stimuli. Scand. J. Gastroent., in pressGoogle Scholar
  3. 3.
    Schaffalitzky de Muckadell OB, Fahrenkrug J, Holst JJ: Release of vasoactive intestinal polypeptide (VIP) by electric stimulation of the vagal nerves. Gastroenterology 72, 373–375, 1977Google Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • J. Fahrenkrug
    • 1
  • O. B. Schaffalitzky De Muckadell
    • 1
  1. 1.Department of Clinical ChemistryBispebjerg HospitalCopenhagenDenmark

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