Radioimmunoassay of Secretin

  • P. L. Rayford
  • A. Schafmayer
  • J. C. Thompson
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 106)


Several sensitive radioimmunoassay systems for secretin have been developed and reported1–6. The development of these assay systems depended upon the generation of a specific secretin antiserum and the iodination of secretin (natural porcine secretin, synthetic secretin or a tyrosyl derivative of secretin). This manuscript reports the results of studies conducted in our laboratory to validate a secretin radioimmunoassay.


Vasoactive Intestinal Peptide Assay Tube Logit Transformation Plasma Secretin Inhibition Line 


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  1. 1.
    Bloom SR, Ogawa O: Radioimmunoassay of human peripheral plasma secretin. J Endocrinol 58: 24, 1973Google Scholar
  2. 2.
    Boden G, Chey WY: Preparation and specificity of antiserum to synthetic secretin and its uses in radioimmunoassay (RIA). Endocrinology 92: 1617, 1973PubMedCrossRefGoogle Scholar
  3. 3.
    Boehm M, Lee Y, Chey WY: Radioimmunoassay of secretin. I. Production of secretin antibodies and development of the radioimmunoassay. In: Endocrinology of the Gut. WY Chey and FP Brooks (eds), Charles B. Slack Inc, Thorofare, NJ, 1974, pp 310–319Google Scholar
  4. 4.
    Rayford PL, Curtis PJ, Fender HR, Thompson JC: Plasma levels of secretin in man and dogs: Validation of a secretin radioimmunoassay. Surgery 79: 658–665, 1976PubMedGoogle Scholar
  5. 5.
    Kolts BE, McGuigan JE: Radioimmunoassay measurement of secretin half-life in man. Gastroenterology 72: 55–60, 1977PubMedGoogle Scholar
  6. 6.
    Straus E, Yalow RS: Fasting and postprandial plasma secretin concentrations. Gastroenterology 72: A160/ 1183, 1977Google Scholar
  7. 7.
    Rodbard D, Rayford PL, Cooper JA, Ross GT: Statistical quality control of radioimmunoassays. J Clin Endocrin Metab 28: 1412–1418, 1968CrossRefGoogle Scholar
  8. 8.
    Chey WY, Hendricks J, Tai HH: Plasma secretin in fasting and postprandial states in man. Gastroenterology 72: A133/ 1156, 1977Google Scholar
  9. 9.
    Fender HR, Curtis PJ, Rayford PL, Thompson JC: Simultaneous bioassay and radioimmunoassay of secretin. Gastroenterology 72: A35/ 1058, 1977Google Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • P. L. Rayford
    • 1
  • A. Schafmayer
    • 1
  • J. C. Thompson
    • 1
  1. 1.Department of SurgeryThe University of Texas Medical BranchGalvestonUSA

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