Motilin-, Substance P- and Somatostatin-Like Immunoreactivities in Extracts from Dog, Tupaia and Monkey Brain and GI Tract

  • C. Yanaihara
  • H. Sato
  • N. Yanaihara
  • S. Naruse
  • W. G. Forssmann
  • V. Helmstaedter
  • T. Fujita
  • K. Yamaguchi
  • K. Abe
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 106)


Motilin, a 22-amino acid polypeptide, has been isolated from porcine duodenal and jejunal mucosa as a principle which exerts motor-stimulating effects on gastrointestinal smooth muscle.1 Dryburgh and Brown2 have developed a radioimmunoassay and demonstrated the increase of immunoreactive motilin in the circulation of the dog by strong duodenal alkalization. Subsequently, the measurement of motilin immunoreactivity in human plasma has been demonstrated3. Using radioimmunoassay technique, the distribution of immunoassayable motilin has been determined in boiling-water extracts of tissues from various parts of the human gastrointestinal tract and other organs. The highest concentration of immunoreactive motilin was found in the duodenum, with little in the colon, liver, pancreas and brain, suggesting that the role of motilin may relate to digestive function3. Histochemical studies4 have also shown that motilin is present in the EC-cells of the mammalian small intestine, mostly in duodenum and jejunum, but not in gastric and lower intestinal EC-cells. Forssmann et al. have demonstrated immunohisto-chemically using antisera against our synthetic motilin that the motilin cells are present predominantly in duodenum and upper jejunum in Tupaia belangeri and human biopsy materials.


Vasoactive Intestinal Polypeptide Posterior Pituitary Minimum Detectable Dose Immunoreactive Substance Respective Hormone 
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Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • C. Yanaihara
    • 1
  • H. Sato
    • 1
  • N. Yanaihara
    • 1
  • S. Naruse
    • 1
  • W. G. Forssmann
    • 2
  • V. Helmstaedter
    • 2
  • T. Fujita
    • 3
  • K. Yamaguchi
    • 4
  • K. Abe
    • 4
  1. 1.Laboratory of Bioorganic ChemistryShizuoka College of PharmacyShizuokaJapan
  2. 2.Department of AnatomyUniversity of HeidelbergGermany
  3. 3.Third Department of Anatomy, School of MedicineUniversity of NiigataNiigataJapan
  4. 4.Endocrine DivisionNational Cancer Center Research InstituteTokyoJapan

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