Regulation of Epidermal Growth Factor (EGF) Binding Sites in Rat Granulosa Cells by Follicle Stimulating Hormone (FSH)

  • H. Fujinaga
  • M. Yamoto
  • T. Shikone
  • K. Nishimori
  • R. Nakano
Part of the Serono Symposia USA book series (SERONOSYMP)


In the present study, we examined the effect of FSH on [125I]mEGF binding to rat granulosa cells (GC). The GC, isolated from diethylstilbestrol-treated immature female rats, were cultured with ovine FSH (NIADDK-oFSH-17) (1–300 ng/mL) in serum-free McCoy’s 5a medium for 48 h at 37°C and then were incubated with [125I]mEGF (0.05 μCi) for 18 h at 20°C to quantify EGF binding sites. After culture with oFSH for 48 h, the GC were cultured with or without mEGF (30 ng/mL) for 48 h at 37°C, then tissue plasminogen activator (tPA) activities in the conditioned media were examined by the fibrin overlay method. FSH significantly increased [125I]mEGF binding to rat GC in a dose-dependent manner, and EGF produced a dose-dependent increase in tPA activity compared to the control. Our findings suggest that functional EGF binding sites in rat GC are regulated by FSH and that FSH might influence folliculogenesis by regulating EGF receptors (EGF-R).


Epidermal Growth Factor Receptor Epidermal Growth Factor Conditioned Medium Granulosa Cell Epidermal Growth Factor Binding 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    St Arnaud R, Walker P, Kelly PA, Labrie F. Rat ovarian epidermal growth factor receptors: characterization and hormonal regulation. Mol Cell Endocrinol 1983; 31: 43–52.CrossRefGoogle Scholar
  2. 2.
    Galway AB, Oikawa M, Ny T, Hsueh AJW. Epidermal growth factor stimulates tissue plasminogen activator and messenger ribonucleic acid levels in cultured rat granulosa cells: mediation by pathways independent of protein kinase-A and -C. Endocrinology 1989; 125: 126–35.PubMedCrossRefGoogle Scholar
  3. 3.
    Carpenter G, Cohen S. 125I-Labeled human epidermal growth factor binding, internalization, and degradation in human fibroblast. J Cell Biol 1976; 71: 159–71.PubMedCrossRefGoogle Scholar
  4. 4.
    Mondschein JS, Schomberg DW. Growth factors modulate gonadotropin receptor induction in granulosa cell cultures. Science 1980; 211: 1179–80.CrossRefGoogle Scholar
  5. 5.
    Jones PBC, Welsh TH, Jr, Hsueh AJW. Regulation of ovarian progestin production by epidermal growth factor in cultured rat granulosa cells. J Biol Chem 1982; 257: 11268–73.PubMedGoogle Scholar
  6. 6.
    Buck PA, Schomberg DW. [125I]Iodo-epidermal growth factor binding and mitotic responsiveness of porcine granulosa cells are modulated by differentiation and follicle-stimulating hormone. Endocrinology 1988; 122: 28–33.PubMedCrossRefGoogle Scholar
  7. 7.
    Feng P, Knecht M, Catt K. Hormonal control of epidermal growth factor receptors by gonadotropins during granulosa cell differentiation. Endocrinology 1987; 120: 1121–6.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York, Inc. 1992

Authors and Affiliations

  • H. Fujinaga
  • M. Yamoto
  • T. Shikone
  • K. Nishimori
  • R. Nakano

There are no affiliations available

Personalised recommendations