Novel Testicular Products of the Human SHBG/ABP Gene

  • Geoffrey L. Hammond
Part of the Serono Symposia USA book series (SERONOSYMP)


The blood of most mammalian species contains a binding protein for the sex-steroid hormones; in humans, this protein is most often referred to as sex-hormone binding globulin (SHBG). Rodents are notable exceptions to this rule, and it is well known that adult rats lack a plasma equivalent of SHBG. A similar protein has, however, been found in fetal rat blood during late gestation and is probably produced by the liver (1). In humans and other primates, the protein exhibits a high affinity for both androgens and estrogens (2), and plasma concentrations of SHBG determine the distribution of these hormones between the various protein-bound and nonprotein-bound fractions in the blood (3). As a result, SHBG influences the bioavailability of sex steroids at the target cell level (4). More recently, evidence has accumulated to support the concept that SHBG may play a more direct role in the actions of sex steroids, and a plasma membrane receptor that interacts specifically with SHBG has been isolated from human prostate tissue (5). This has resulted in a reevaluation of the physiological role of SHBG in a recent comprehensive review of the subject (6).


Androgen Binding Protein Human Testis cDNA Library Testicular Product Human Prostate Tumor Cell Line Target Cell Level 
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  1. 1.
    Gunsalus GL, Carreau S, Vogel DL, Musto NA, Bardin CW. Sexual differentiation: basic and clinical aspects. In: Serio M, ed. New York: Raven Press, 1984: 53–64.Google Scholar
  2. 2.
    Renoir J-M, Mercier-Bodard C, Baulieu E-E. Hormonal and immunological aspects of the phylogeny of sex steroid binding plasma protein. Proc Natl Acad Sci USA 1980; 77: 4578–82.PubMedCrossRefGoogle Scholar
  3. 3.
    Anderson DC. Sex-hormone binding globulin. Clin Endocrinol (0x0 1974; 3: 69–96.CrossRefGoogle Scholar
  4. 4.
    Siiteri PK, Murai JT, Hammond GL, Nisker JA, Raymoure WJ, Kuhn RW. The serum transport of steroid hormones. Recent Prog Horm Res 1982; 38: 457–510.PubMedGoogle Scholar
  5. 5.
    Hryb DJ, Khan MS, Romas MA, Rosner W. Solubilization and partial characterization of sex hormone-binding globulin receptor from human prostate. J Biol Chem 1989; 264: 5378–83.PubMedGoogle Scholar
  6. 6.
    Rosner, W. The functions of corticosteroid-binding globulin and sex hormone-binding globulin: recent advances. Endocr Rev 1990; 11: 80–91.PubMedCrossRefGoogle Scholar
  7. 7.
    French FS, Ritzen EM. A high-affinity androgen binding protein (ABP) in rat testis: evidence for secretion into efferent duct fluid and absorption by epididymis. Endocrinology 1973; 93: 88–95.PubMedCrossRefGoogle Scholar
  8. 8.
    Bardin CW, Musto N, Gunsalus G, et al. Extracellular androgen binding proteins. Annu Rev Physiol 1981; 43: 189–98.PubMedCrossRefGoogle Scholar
  9. 9.
    Pelliniemi LT, Dym M, Gunsalus GL, Musto NA, Bardin CW, Fawcett DW. Immunocytochemical localization of androgen-binding protein in the male reproductive tract. Endocrinology 1981; 108: 925–31.PubMedCrossRefGoogle Scholar
  10. 10.
    Cheng CY, Musto NA, Gunsalus GL, Frick J, Bardin CW. There are two forms of androgen binding protein in human testes. J Biol Chem 1985; 260: 5631–40.PubMedGoogle Scholar
  11. 11.
    Cheng CY, Frick J, Gunsalus GL, Musto NA, Bardin CW. Human testicular androgen-binding protein shares immunodeterminants with serum testosteroneestradiol-binding globulin. Endocrinology 1984; 114: 1395–401.PubMedCrossRefGoogle Scholar
  12. 12.
    Hammond GL, Robinson PA, Sugino H, Ward DN, Finne J. Physicochemical characteristics of human sex hormone binding globulin: evidence for two identical subunits. J Steroid Biochem 1986; 24: 815–24.PubMedCrossRefGoogle Scholar
  13. 13.
    Hsu AF, Troen P. An androgen binding protein in the testicular cytosol of human testis. Comparison with human plasma testosterone-estrogen binding globulin. J Clin Invest 1978; 61: 1611–9.PubMedCrossRefGoogle Scholar
  14. 14.
    Cheng CY, Gunsalus GL, Musto NA, Bardin CW. The heterogeneity of rat androgen-binding protein in serum differs from that in testis and epididymis. Endocrinology 1984; 114: 1386–94.PubMedCrossRefGoogle Scholar
  15. 15.
    Tindall DJ, Means AR. Properties and hormonal regulation of androgen binding proteins. In: Thomas JA, Singhal RL, eds. Advances in sex hormone research; vol 4. Baltimore: Urban and Schwarzenberg, 1980: 295–327.Google Scholar
  16. 16.
    Joseph DR, Hall SH, Conti M, French FS. The gene structure of rat androgen-binding protein: identification of potential regulatory deoxyribonucleic acid elements of a follicle-stimulating hormone-regulated protein. Mol Cell Endocrinol 1988; 2: 3–13.CrossRefGoogle Scholar
  17. 17.
    Reventos J, Hammond GL, Crozat A, et al. Hormonal regulation of rat androgen-binding protein (ABP) messenger ribonucleic acid and homology of human testosterone-estradiol-binding globulin and ABP complementary deoxyribonucleic acids. Mol Cell Endocrinol 1988; 2: 125–32.CrossRefGoogle Scholar
  18. 18.
    Skinner MK, Fritz IB. Testicular peritubular cells secrete a protein under androgen control that modulates Sertoli cell functions. Proc Natl Acad Sci USA 1985; 82: 114–8.PubMedCrossRefGoogle Scholar
  19. 19.
    Joseph DR, Sullivan PM, Fenstermacher DA, Behrendsen ME, Zahnow CA. Alternate processed androgen-binding protein mRNAs; identification of a fusion transcript with DOPA decarboxylase-like RNA [Abstract]. Proc 71st annu meet Endocr Soc. Seattle, WA, 1989: 71.Google Scholar
  20. 20.
    Hammond GL, Underhill DA, Smith CL, et al. The cDNA-deduced primary structure of human sex hormone-binding globulin and location of its steroid-binding domain. FEBS Lett 1987; 215: 100–4.PubMedCrossRefGoogle Scholar
  21. 21.
    Joseph DR, Hall SH, French FS. Rat and androgen-binding protein: evidence for identical subunits and amino acid sequence homology with human sex hormone-binding globulin. Proc Natl Acad Sci USA 1987; 84: 339–43.PubMedCrossRefGoogle Scholar
  22. 22.
    Hammond GL, Underhill DA, Rykse H, Smith CL. The human sex hormone binding globulin gene contains exons for androgen binding protein and two other testicular messenger RNAs. Mol Cell Endocrinol 1989; 3: 1869–76.CrossRefGoogle Scholar
  23. 22.
    Hammond GL, Underhill DA, Rykse H, Smith CL. The human sex hormone binding globulin gene contains exons for androgen binding protein and two other testicular messenger RNAs. Mol Cell Endocrinol 1989; 3: 1869–76.CrossRefGoogle Scholar
  24. 22.
    Hammond GL, Underhill DA, Rykse H, Smith CL. The human sex hormone binding globulin gene contains exons for androgen binding protein and two other testicular messenger RNAs. Mol Cell Endocrinol 1989; 3: 1869–76.CrossRefGoogle Scholar
  25. 25.
    Hammond GL. Molecular properties of corticosteroid binding globulin and the sex-steroid binding proteins. Endocr Rev 1990; 11: 65–79.PubMedCrossRefGoogle Scholar
  26. 26.
    Monaci P, Nicosia A, Cortese R. Two different liver-specific factors stimulate in vitro transcription from the human al-antitrypsin promoter. EMBO J 1988; 7: 2075–87.PubMedGoogle Scholar
  27. 27.
    Wang Y-M, Bayliss DA, Milthorn DE, Seroogy KB, Joseph DR. The androgen-binding protein gene is expressed in rat brain. [Abstract 195]. 71st annu meet Endocr Soc. Seattle, WA, 1989.Google Scholar
  28. 28.
    Plymate SR. Effects of sex hormone binding globulin (SHBG) on human prostate carcinoma [Abstract 109]. J Steroid Biochem 1990; 36: 40S.Google Scholar

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© Springer-Verlag New York, Inc. 1992

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  • Geoffrey L. Hammond

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