Cell-Cell Interactions that Influence FSH Regulation of Testis Function

  • John N. Norton
  • Catherine Tananis Anthony
  • Michael K. Skinner
Part of the Serono Symposia USA book series (SERONOSYMP)


Follicle stimulating hormone (FSH) is a gonadotropin secreted from the pituitary that influences the function and differentiation of the testicular Sertoli cell (Sc). This influence of FSH on Sc is mediated through activation of adenylate cyclase, followed by elevation of cAMP levels, stimulation of cAMP-dependent protein kinase, and regulation of gene expression (1–5). FSH promotes and regulates Sc function and differentiation throughout pubertal development; however, the role of FSH in the adult animal is unclear and remains to be fully elucidated.


Nerve Growth Factor Follicle Stimulate Hormone Sertoli Cell Leydig Cell Germinal Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Murad F, Strauch BS, Vaughn M. The effect of gonadotrophins on testicular adenylate cyclase. Biochim Biophys Acta 1969; 177: 591–8.PubMedCrossRefGoogle Scholar
  2. 2.
    Kuehl F, Patanelli DJ, Humes JL, Tarnoff J. Testicular adenylate cyclase: stimulation by the pituitary gonadotrophins. Biol Reprod 1970; 2: 153–63.CrossRefGoogle Scholar
  3. 3.
    Means AR, Hall PF. Effect of FSH on protein biosynthesis in testes of the immature rat. Endocrinology 1967; 81: 1151–60.PubMedCrossRefGoogle Scholar
  4. 4.
    Means AR, MacDougall E, Soderling T, Corbin JD. Testicular adenosine 3’,5’monophosphate-dependent protein kinase. J Biol Chem 1974; 249: 1231–8.PubMedGoogle Scholar
  5. 5.
    Means AR. Concerning the mechanism of FSH action: rapid stimulation of testicular synthesis of nuclear RNA. Endocrinology 1971; 89: 981–9.PubMedCrossRefGoogle Scholar
  6. 6.
    Janecki A, Jakubowiak A, Lukaszyk A. Stimulatory effect of Sertoli cell secretory products on testosterone secretion by purified Leydig cells in primary culture. Mol Cell Endocrinol 1985; 42: 235–43.PubMedCrossRefGoogle Scholar
  7. 7.
    Wattenberg LW. Microscopic histochemical demonstration of steroid-3-oldehydrogenase in tissue sections. J Histochem Cytochem 1958; 6: 225–32.PubMedCrossRefGoogle Scholar
  8. 8.
    Clermont Y, Harvey SG. Duration of the cycle of the seminiferous epithelium of normal, hypophysectomized and hypophysectomized-hormone treated albino rats. Endocrinology 1965; 76: 80–9.PubMedCrossRefGoogle Scholar
  9. 9.
    Sanborn BM, Steinberger A, Meistrich ML, Steinberger E. Androgen binding sites in testis cell fractions as measured by a nuclear exchange assay. J Steroid Biochem 1975; 6: 1459–65.PubMedCrossRefGoogle Scholar
  10. 10.
    Skinner MK, Griswold MD. Secretion of testicular transferrin by cultured Sertoli cells is regulated by hormones and retinoids. Biol Reprod 1982; 27: 211–21.PubMedCrossRefGoogle Scholar
  11. 11.
    Margions AN, Liotta A, Vaudry H, Bardin CW, Krieger DT. Characterization of immunoreactive proopiomelanocortin-related peptides in rat testis. Endocrinology 1983; 113: 663–71.CrossRefGoogle Scholar
  12. 12.
    Boitani C, Mather JP, Bardin CW. Stimulation of adenosine 3’,5’-monophosphate production in rat Sertoli cells by a-melanotropin-stimulating hormone (aMSH) and desacetyl aMSH. Endocrinology 1986; 118: 1513–8.PubMedCrossRefGoogle Scholar
  13. 13.
    Morris D, Vola WW, Bardin CW. 3-endorphin regulation of FSH-stimulated inhibin production is a component of a short loop system in rats. Biochem Biophys Res Commun 1987; 148: 1513–9.PubMedCrossRefGoogle Scholar
  14. 14.
    Galdieri M, Monaco L, Stefanini M. Secretion of androgen binding protein by Sertoli cells is influenced by contact with germ cells. J Androl 1984; 5: 409–15.PubMedGoogle Scholar
  15. 15.
    Le Magueresse B, Jegou B. In vitro effects of germ cells on the secretory activity of Sertoli cells recovered from rats of different ages. Endocrinology 1988; 122: 1672–80.PubMedCrossRefGoogle Scholar
  16. 16.
    Le Magueresse B, Jegou B. Possible involvement of germ cells in the regulation of oestradiol-173 and ABP secretion by immature rat Sertoli cells (in vitro studies). Biochem Biophys Res Comm 1986; 141: 861–9.PubMedCrossRefGoogle Scholar
  17. 17.
    Ayer-LeLievre C, Olson L, Ebendal T, Hallbook F, Persson H. Nerve growth factor mRNA and protein in the testis and epididymis of mouse and rat. Proc Natl Acad Sci USA 1988; 85: 2628–32.PubMedCrossRefGoogle Scholar
  18. 18.
    Persson H, Ayer-LeLievre C, Soder O, et al. Expression of 3-nerve growth factor receptor mRNA in Sertoli cells downregulated by testosterone. Science 1990; 247: 704–7.PubMedCrossRefGoogle Scholar
  19. 19.
    Skinner MK, Takacs K, Coffey RJ. Cellular localization of transforming growth factor-a gene expression and action in the seminiferous tubule: peritubular cellSertoli cell interactions. Endocrinology 1989; 124: 845–54.PubMedCrossRefGoogle Scholar
  20. 20.
    Skinner MK, Moses HL. Transforming growth factor-E3 gene expression and action in the seminiferous tubule: peritubular cell-Sertoli cell interactions. Mol Cell Endocrinol 1989; 3: 625–34.CrossRefGoogle Scholar
  21. 21.
    Suarez-Quian CA, Dai M, Onoda M, Kriss RM, Dym M. Epidermal growth factor receptor localization in the rat and monkey testes. Biol Reprod 1989; 41: 921–32.PubMedCrossRefGoogle Scholar
  22. 22.
    Benahmed M, Esposito G, Sordoillet C, et al. Transforming growth factor-13 and its related peptides in the testis: an intragonadal polypeptide control system. Persp Androl 1989; 53: 191.Google Scholar
  23. 23.
    Skinner MK, Fritz 1B. Testicular peritubular cells secrete a protein under androgen control that modulates Sertoli cell functions. Proc Natl Acad Sci USA 1985; 82: 114–8.PubMedCrossRefGoogle Scholar
  24. 24.
    Skinner MK, Fetterolf PM, Anthony CT. Purification of the paracrine factor, PMod-S, produced by testicular peritubular cells that modulates Sertoli cell function. J Biol Chem 1988; 263: 2884–90.PubMedGoogle Scholar
  25. 25.
    Norton JN, Skinner MK. Regulation of Sertoli cell function and differentiation through the actions of a testicular paracrine factor P-Mod-S. Endocrinology 1989; 124: 2711–9.PubMedCrossRefGoogle Scholar
  26. 26.
    Francis GL, Brown TJ, Bercu BB. Regulation by homologous hormone exposure. Biol Reprod 1981; 24: 955–61.PubMedCrossRefGoogle Scholar
  27. 27.
    Le Gac F, Attramadal H, Jahnsen T, Hansson V. Studies on the mechanism of follicle-stimulating hormone-induced desensitization of Sertoli cell adenyl cyclase in vitro. Biol Reprod 1985; 32: 916–24.PubMedCrossRefGoogle Scholar
  28. 28.
    Conti M, Geremia R, Adamo S, Stefanini M. Regulation of Sertoli cell cyclic adenosine 3’,5’-monophosphate phosphodiesterase activity by follicle stimulating hormone and dibutyryl cyclic AMP. Biochem Biophy Res Commun 1981; 98: 1044–50.CrossRefGoogle Scholar
  29. 29.
    Migliaccio S, Conti M. Long term treatment with adenosine analogs modifies the responsiveness of immature rat Sertoli cell in culture. Endocrinology 1990; 126: 134–41.PubMedCrossRefGoogle Scholar
  30. 30.
    Monaco L, Adamo S, Stefanini M, Conti M. Signal transduction in the Sertoli cell: serum modulation of the response to FSH. J Steroid Biochem 1989; 32: 129–34.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York, Inc. 1992

Authors and Affiliations

  • John N. Norton
  • Catherine Tananis Anthony
  • Michael K. Skinner

There are no affiliations available

Personalised recommendations