Regulation of the LH/CG Receptor by Gonadotropins

  • Deborah L. Segaloff
  • JoAnne S. Richards
  • Mario Ascoli
  • Haiyun Wang
Part of the Serono Symposia USA book series (SERONOSYMP)


The induction of the lutropin/choriogonadotropin receptor (LH/CG-R) by follicle-stimulating hormone (FSH) is one of the hallmarks of the differentiating granulosa cell (GC) (1–3). The mechanisms underlying the induction and regulation of the LH/CG-R, however, are for the most part not well understood. Utilizing cultured GC, many different laboratories have characterized the FSH-dependent induction of LH/CG-R as defined by hCG binding activity. From these experiments, it has been possible to make the following conclusions. (1) The induction of the LH/CG-R by FSH is mediated by the FSH-induced increase in intracellular cAMP (4–7). Thus, other agents that increase intracellular cAMP, such as cholera toxin, forskolin, or exogenously added cAMP derivatives, are capable of mimicking the induction of the LH/CG-R. (2) The dose-response curve for the induction of the LH/CG-R by FSH or agents that increase cAMP is bell shaped, such that at high concentrations of hormone or cAMP, the induction is submaximal (5, 8, 9). (3) The time course of this induction process is relatively slow, with increased hCG binding activity in cells cultured with FSH observed only 24 h after the incubation has been initiated. Maximal binding is typically observed at 72–96 h (5, 8–10). (4) The induction and the maintenance of the LH/CG-R in GC requires the continual presence of FSH. If FSH is removed at any time, the levels of LH/CG-R return to basal within 24 h (4). Thus, the induction process does not involve a “committed step,” but is a continuous process. (5) The FSH-dependent increase in cell-surface LH/CG-R in GC is not due to the mobilization of an intracellular pool of preformed receptors to the cell surface (11). (6) The FSH-dependent increase in LH/CG-R can be prevented when protein synthesis is inhibited (11).


Luteinizing Hormone Granulosa Cell mRNA Content Luteinizing Hormone Receptor Leydig Tumor Cell 
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Copyright information

© Springer-Verlag New York, Inc. 1992

Authors and Affiliations

  • Deborah L. Segaloff
  • JoAnne S. Richards
  • Mario Ascoli
  • Haiyun Wang

There are no affiliations available

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