New Approaches for Agricultural Molecular Biology: From Single Cells to Field Analysis
Although the advent of molecular biology and recombinant DNA technologies has caused tremendous excitement and enthusiasm among many scientists and policy-makers whose goal is the improvement of agriculture through research, the realities of the time scale over which these technologies must be applied, and the extent to which they are currently inadequate is now being appreciated. The main reason for these inadequacies is simply our overwhelming ignorance of the biology that underlies agriculture. Traditional crop manipulation, as typified by plant breeding, is an empirical craft that is not generally based on a firm understanding of the processes that govern the characters being manipulated. Although the methodology used in an advanced plant breeding program can be quite sophisticated, it is fundamentally limited by the need to visualize and select for traits from existing variation. The complexity of agriculture and the urgency of its success or failure has generally required this empirical approach to its manipulation, while the power of modern molecular biology lies in the acquisition of an understanding of the processes by experimental manipulation.
KeywordsGene Fusion Transcriptional Fusion Fusion Genetic Modern Molecular Biology Plant Science Research
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- Dutton, G.J., ed., 1966, Glucuronic Acid, Free and Combined, Academic Press, New York.Google Scholar
- Dutton, G.J., 1980, Glucuronidation of Drugs and Other Compounds, CRC Press, Boca Raton, Florida.Google Scholar
- Jefferson, R.A.,1985, DNA Transformation of Caenorhabditis elegans: Development and Application of a New Gene Fusion System, Ph.D. Dissertation, University of Colorado at Boulder.Google Scholar
- Jefferson, R.A., 1988, Plant report genes: the GUS gene fusion system, In: “Genetic Engineering, Vol. 10,” J.K. Setlow, ed., Plenum Press, New York.Google Scholar
- Levvy, G.A. and Conchie, J. 1966, Å-Glucuronidase and the hydrolysis of glucuronides, In: “Glucuronic Acid, Free and Combined,” G.J. Dutton, ed., Academic Press, New York, 301.Google Scholar
- Masson, P., and Fedoroff, N.V.,1989, Mobility of the maize Suppressor-mutator element in transgenic tobacco cells, Proc. Natl. Acad. Sci. USA, 86: 2219–2223.Google Scholar
- Miller J.H., Reznikoff, W.S., Silverstone, A.E., Ippen, K., Signer, E.R., and Beckwith, J.R.,1970, Fusions of the lac and trp regions of the Escherichia coli chromosome, J. Bacteriol., 104: 12–73.Google Scholar
- Park, W.D., 1986, In: “Potato Physiology,” P. Li., ed., Academic Press.Google Scholar
- Schmitz, U.K., Jefferson R.A., and Lonsdale, D.M., 1989, GUS as a gene fusion marker in the yeast, Saccharomyces cerevisiae, Curr Genet., In press.Google Scholar
- Stoeber, F.,1957, Sur la biosynthese induite de la A-glucuronidase chez Escherichia coli, C.R. Acad. Sci., 244: 950.Google Scholar
- Stoeber, F., 1961, Etudes des proprietes et de la biosynthese de la glucuronidase et de la glucuronide-permease chez Escherichia coli, These de Docteur es Sciences, Paris.Google Scholar