Using Plant Virus and Related RNA Sequences to Control Gene Expression
Plant virus replication relies on the biochemistry of its host cell, strongly suggesting that viral gene expression closely mimics the host’s own mechanism of gene expression. This fundamental principle has been a driving force behind research directed at understanding the molecular biology of viruses, using them as a tool for studying both viral and host gene expression. Many recent advances have been made possible by recombinant DNA technology, which has allowed a more thorough analysis of basic viral genome structure and function. Armed with this basic knowledge, virologists have been successful in manipulating and using viral genomes and related sequences to alter gene expression and thereby altering phenotypes. Bacterial, animal, and plant viruses have all been subjected to this approach.
KeywordsTobacco Mosaic Virus Cucumber Mosaic Virus Plant Virus Control Gene Expression Chloramphenicol Acetyl Transferase
Unable to display preview. Download preview PDF.
- Gerlach, W. L., Miller, W. A., and Waterhouse, P. M., 1987, Molecular genetics of barley yellow drawf virus, Barley Yellow Dwarf Newsletter, 1: 17–19.Google Scholar
- Haseltine, W. A., and Wong-Staal, F., 1988, The molecular biology of the AIDS virus, Scientific American, 34–42.Google Scholar
- Schneider, I. R., 1977, Defective plant viruses, in: “Bletsville Symposia on Agricultural Research-Virology in Agriculture”, J. R. Romberger, ed., Allenheld Osmun, New Jersey (1977), pp. 201–219.Google Scholar
- Turner, N. E., O’Connell, K. M., Nelson, R. S., Sanders, P. R., Beachy, R. N., Fraley, R. T., and Shah, D. M., 1987, Expression of alfalfa mosaic virus coat protein gene confers crossprotection in transgenic tobacco and tomato plants, EMBO J., 6: 1181–1187.Google Scholar