Genetic Transformation of Maize Cells by Particle Bombardment and the Influence of Methylation on Foreign-Gene Expression
Much of the progress in understanding plant gene regulation has been due to the availability of gene transfer systems for many plant species. The results from these studies of the structure of plant gene regulatory regions have been reviewed recently (Schell, 1987; Willmitser, 1988). Additionally, the development of techniques for transferring genes into crop plants allows new agronomic traits to be introduced (Goodman et al., 1987). Recently, resistance to insects (Fischhoff et al., 1987; Hilder et al., 1987; Vaeck et al., 1987), viruses (Abel et al., 1986; Gerlach et al., 1987; Harrison et al., 1987; Cuozzo et al., 1987; Nelson et al., 1987), and herbicides (Shah et al., 1986; della-Cioppa et al., 1987; Fillatti et al., 1987; Cheung et al., 1988) have been introduced into crop plants via gene transfer techniques. Unfortunately much of this progress has not benefited cereals, as general techniques for transferring genes into cereal plants, such as rice (Oryza sativa), wheat (Triticum aestivum) and maize (Zea mays), are not as advanced as for many of the dicot plants.
KeywordsParticle Bombardment Adh1 Gene Maize Cell Adh1 Promoter Intact Copy
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