Transformation and Regeneration of Important Crop Plants: Rice as the Model System for Monocots
The use of transgenic plants as experimental tools in gene analysis and for the alteration of agronomic characters is fundamental to our attempts to improve crops. There are at least five ways to introduce foreign genes into plants. Here we would like to describe the merits and shortcomings of each method and present our results in producing transgenic rice plants. In addition, we propose a method using transgenic plants to facilitate cloning of genes which are difficult to identify by other methods.
KeywordsTransgenic Plant Foreign Gene Transgenic Rice Plant Restriction Fragment Length Polymorphism Analysis Recognition Protein
Unable to display preview. Download preview PDF.
- Botstein, D., White, R. L., Skolnick, M., and Davis, R. W., 1980, Construction of a genetic linkage map in man using restriction fragment length polymorphisms, Am. J. Human Genet., 32: 314–331.Google Scholar
- Clarke, L., and Carbon I., 1979, Selection of specific clones from colony banks by suppression or complementation tests, Methods in Enzymol., 68: 346–408.Google Scholar
- Duan, X., and Chen, S., 1985, Variation of the characters in rice (Oryza sativa) induced by foreign DNA uptake, China Agricul. Sci., 3: 6–9.Google Scholar
- Lillis, M., and Freeling, M., 1986, Mu transposons in maize, TIG, July: 183–187.Google Scholar
- Nagao, S., Takahashi, M., and Kinoshita, T., 1968, Heterotic effect of alleles at Pl-locus in rice plant, Genetic studies on rice plant, XXX, J. Fac. Agr. Hokkaido Univ., 56 (1): 45–56.Google Scholar
- Oka, H. I., and Khush, G. S., eds., 1984, Rice Genetics Newsletter, Vol. 1, Japanese Rice Genetics Information Committee, Japan (1984).Google Scholar
- O’Reilley, C., Shepherd, N. S., Pereira, A., Schwarz-Sommers, Z., Bertram, I., and Peterson, P. A., 1985, Molecular cloning of the al locus of Zea mays using the transposable elements En and Mul, EMBO J., 4: 877–882.Google Scholar
- Wu, R., 1988, Methods for transforming plant cells, in: “Plant Biotechnology,” S.D. Kung, and C.J. Arntzen, eds., Butterworth Publishers ( 1988 ), pp. 35–51.Google Scholar
- Zhang, H. M., Yang, H., Rech, E. L., Golds, T. J., Davis, A. S., Mulligan, B. J., Cocking, E. C., and Davey, M. R., 1988, Transgenic rice plants produced by electroporation mediated plasmid uptake into protoplasts, Plant Cell Reports, 7: 379–384.Google Scholar