Haploids in Cereal Improvement: Anther and Microspore Culture

  • K. J. Kasha
  • A. Ziauddin
  • U.-H. Cho
Part of the Stadler Genetics Symposia Series book series (SGSS)


For years, cereals and monocots in general have been poor responders in in vitro haploid production systems when compared to many dicot crops. The response frequency was low with very strong genotype effects and often large numbers of albino progeny. However, we are now seeing evidence of a breakthrough that can provide embryo frequencies equivalent to those obtained in some Brassicas and other dicot crops. These results are with specific genotypes of barley (Hordeum vulgare L.) (Hunter, 1988; Hunter et al., 1989; Olsen, 1987), where green plant production has increased up to 100 fold. As a result, further improvements in wheat (Triticum aestivum L.) and other cereals will likely follow. In addition, plants have been obtained from isolated microspore culture in barley, wheat, rice (Oryza sativa L.) and corn (Zea mays L.). These cultures open up new possibilities for transformation, mutation and selection in cereals.


Green Plant Anther Culture Microspore Culture Cold Pretreatment Albino Plant 
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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • K. J. Kasha
    • 1
  • A. Ziauddin
    • 1
  • U.-H. Cho
    • 1
  1. 1.Crop Science Dept.University of GuelphGuelphCanada

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